tissue: Stomach strain: mixed (129X1/Sv / C57BL/6) Sex: chimeric mice with male ES cells
Extracted molecule
total RNA
Extraction protocol
RNeasy Plus Mini kit
Label
Biotin
Label protocol
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
Hybridization protocol
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99℃ for 5 minutes, then 45℃ for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 80μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 17 hours at 45° at 60rpm• Fluidics washing is FS450_0007
Scan protocol
Affymetrix Gene ChIP Scanner 3000 TG
Data processing
Data analyses were processed using Genespring software (version13.0). For the analysis, C-OSKM NoDox pancreas-1_(MoGene-1_0-st-v1).CEL(raw), C-OSKM Dox3d pancreas-1_(MoGene-1_0-st-v1).CEL(raw), KC-OSKM NoDox Stomach-1_(MoGene-1_0-st-v1).CEL(raw), KC-OSKM NoDox Stomach-2_(MoGene-1_0-st-v1).CEL(raw), KC-OSKM NoDox Stomach-3_(MoGene-1_0-st-v1).CEL(raw), KC-OSKM 1w3w Stomach-1_(MoGene-1_0-st-v1).CEL(raw), KC-OSKM 2w2w Stomach-1_(MoGene-1_0-st-v1).CEL(raw), KC-OSKM 2w2w Stomach-2_(MoGene-1_0-st-v1).CEL(raw),were loaded using Genespring. Summarization algorithm is RMA-16.
