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Sample GSM2693926 Query DataSets for GSM2693926
Status Public on Jul 30, 2019
Title ADPKD patient 1
Sample type RNA
 
Source name ADPKD patient renal cyst tissue
Organism Homo sapiens
Characteristics disease state: ADPKD
tissue: kidney
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from kidney tissue samples of non-ADPKD and ADPKD patients using a Trizol method according to the manufacturer’s instructions (Invitrogen Life Technologies).
Label none
Label protocol Per RNAsample, 100 ng were used as input into the Agilent procedure as recommended by protocol (miRNA Microarray System with miRNA Complete Labeling and Hyb Kit protocol 2.1).
 
Hybridization protocol 100 ng of total RNA was used as an input into the labeling reaction, and labeled miRNA was hybridized to the array for 20 h at 55°C and 20 rpm, as described in the manufacturer’s protocol. After hybridization, the chips were washed and scanned using the Agilent DNA Microarray Scanner.
Scan protocol Global miRNA gene expression analyses were performed on the Agilent Human miRNA Microarray Version 3 software (Agilent design IDs 021827) according to the manufacturer’s instructions (miRNA Microarray System with miRNA Complete Labeling and Hyb Kit protocol 2.1).
Description patients with ADPKD undergoing nephrectomy
PKD_Patient_rep1
Data processing The scanned images were analyzed with GeneSpring GX11 TMEV4.1.
 
Submission date Jul 05, 2017
Last update date Jul 30, 2019
Contact name Sumin Oh
E-mail(s) kstori3924@gmail.com
Phone 82-10-7203-2307
Organization name Sookmyung Women's University
Department Department of Biological Science
Lab LBG
Street address Cheongpa-ro 47-gil 100, Yongsan-gu
City Seoul
ZIP/Postal code 04310
Country South Korea
 
Platform ID GPL18743
Series (1)
GSE100812 Expression patterns of key miRNAs in end-stage ADPKD kidneys

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
miRNABrightCorner30 7.83464
DarkCorner 0.026526531
hsa-miR-720 13.400856
hsa-miR-587 0.026526531
hsa-miR-1236 0.026526531
hsa-miR-607 0.026526531
hsa-miR-1253 0.026526531
hsa-miR-369-3p 0.026526531
hsa-miR-657 0.026526531
hsa-miR-526b 1.8758674
hsa-miR-515-3p 0.026526531
hsa-miR-586 0.026526531
hsa-miR-485-5p 0.026526531
hsa-miR-507 0.026526531
hsa-miR-551b* 0.026526531
hsa-miR-326 0.026526531
hsa-miR-146b-3p 0.026526531
hsa-miR-1283 0.026526531
hsa-miR-622 2.073194
hsa-miR-556-5p 0.026526531

Total number of rows: 961

Table truncated, full table size 22 Kbytes.




Supplementary file Size Download File type/resource
GSM2693926_PKD_Patient_rep1.txt.gz 894.3 Kb (ftp)(http) TXT
Processed data included within Sample table

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