|Public on Jul 30, 2019
|non-ADPKD kidney tissue
|disease state: non-ADPKD
|Total RNA was isolated from kidney tissue samples of non-ADPKD and ADPKD patients using a Trizol method according to the manufacturer’s instructions (Invitrogen Life Technologies).
|Per RNAsample, 100 ng were used as input into the Agilent procedure as recommended by protocol (miRNA Microarray System with miRNA Complete Labeling and Hyb Kit protocol 2.1).
|100 ng of total RNA was used as an input into the labeling reaction, and labeled miRNA was hybridized to the array for 20 h at 55°C and 20 rpm, as described in the manufacturer’s protocol. After hybridization, the chips were washed and scanned using the Agilent DNA Microarray Scanner.
|Global miRNA gene expression analyses were performed on the Agilent Human miRNA Microarray Version 3 software (Agilent design IDs 021827) according to the manufacturer’s instructions (miRNA Microarray System with miRNA Complete Labeling and Hyb Kit protocol 2.1).
|non-ADPKD renal tissue samples were obtained from patients undergoing surgery for clear cell renal cell carcinoma, where malignant cell infiltration was excluded by histology.
|The scanned images were analyzed with GeneSpring GX11 TMEV4.1.
|Jul 05, 2017
|Last update date
|Jul 30, 2019
|Sookmyung Women's University
|Department of Biological Science
|Cheongpa-ro 47-gil 100, Yongsan-gu
|Expression patterns of key miRNAs in end-stage ADPKD kidneys