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Sample GSM2693923 Query DataSets for GSM2693923
Status Public on Jul 30, 2019
Title non-ADPKD 1
Sample type RNA
Source name non-ADPKD kidney tissue
Organism Homo sapiens
Characteristics disease state: non-ADPKD
tissue: kidney
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from kidney tissue samples of non-ADPKD and ADPKD patients using a Trizol method according to the manufacturer’s instructions (Invitrogen Life Technologies).
Label none
Label protocol Per RNAsample, 100 ng were used as input into the Agilent procedure as recommended by protocol (miRNA Microarray System with miRNA Complete Labeling and Hyb Kit protocol 2.1).
Hybridization protocol 100 ng of total RNA was used as an input into the labeling reaction, and labeled miRNA was hybridized to the array for 20 h at 55°C and 20 rpm, as described in the manufacturer’s protocol. After hybridization, the chips were washed and scanned using the Agilent DNA Microarray Scanner.
Scan protocol Global miRNA gene expression analyses were performed on the Agilent Human miRNA Microarray Version 3 software (Agilent design IDs 021827) according to the manufacturer’s instructions (miRNA Microarray System with miRNA Complete Labeling and Hyb Kit protocol 2.1).
Description non-ADPKD renal tissue samples were obtained from patients undergoing surgery for clear cell renal cell carcinoma, where malignant cell infiltration was excluded by histology.
Data processing The scanned images were analyzed with GeneSpring GX11 TMEV4.1.
Submission date Jul 05, 2017
Last update date Jul 30, 2019
Contact name Sumin Oh
Phone 82-10-7203-2307
Organization name Sookmyung Women's University
Department Department of Biological Science
Street address Cheongpa-ro 47-gil 100, Yongsan-gu
City Seoul
ZIP/Postal code 04310
Country South Korea
Platform ID GPL18743
Series (1)
GSE100812 Expression patterns of key miRNAs in end-stage ADPKD kidneys

Data table header descriptions
VALUE Normalized signal intensity

Data table
miRNABrightCorner30 8.435258
DarkCorner 0.026526531
hsa-miR-720 14.653843
hsa-miR-587 0.026526531
hsa-miR-1236 0.026526531
hsa-miR-607 0.026526531
hsa-miR-1253 0.026526531
hsa-miR-369-3p 0.026526531
hsa-miR-657 0.026526531
hsa-miR-526b 0.026526531
hsa-miR-515-3p 0.026526531
hsa-miR-586 0.026526531
hsa-miR-485-5p 0.026526531
hsa-miR-507 6.258451
hsa-miR-551b* 0.026526531
hsa-miR-326 1.2966503
hsa-miR-146b-3p 0.026526531
hsa-miR-1283 0.026526531
hsa-miR-622 0.026526531
hsa-miR-556-5p 0.026526531

Total number of rows: 961

Table truncated, full table size 22 Kbytes.

Supplementary file Size Download File type/resource
GSM2693923_PKD_Normal_rep1.txt.gz 893.2 Kb (ftp)(http) TXT
Processed data included within Sample table

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