|
Status |
Public on Jul 05, 2017 |
Title |
RA_2_BM |
Sample type |
RNA |
|
|
Source name |
Bone marrow Monocytes from RA patient
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: Rheumatoid arthritis (RA) tissue: Bone marrow gender: female
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Rneasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
100 ng of total RNA was amplified and labelled using the GeneChip® one-cycle target labelling and control reagents (Affymetrix)
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|
|
Hybridization protocol |
50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
Data processing |
Data were processed with GCOS 1.4 (TGT = 150) and subsequently imported and further analyzed in the online database (www.bioretis.de)
|
|
|
Submission date |
Jul 04, 2017 |
Last update date |
Jan 23, 2018 |
Contact name |
Biljana Smiljanovic |
E-mail(s) |
biljana.smiljanovic@charite.de
|
Phone |
+49 30 450 513 296
|
Organization name |
Charité - Universitätsmedizin Berlin
|
Department |
Department of Rheumatology and Clinical Immunology
|
Street address |
Charitéplatz 1
|
City |
Berlin |
ZIP/Postal code |
10117 |
Country |
Germany |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE100786 |
Expression data of bone marrow and blood monocytes from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) |
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