|
| Status |
Public on Jul 05, 2017 |
| Title |
RA_2_BM |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow Monocytes from RA patient
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Rheumatoid arthritis (RA)
tissue: Bone marrow
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Rneasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100 ng of total RNA was amplified and labelled using the GeneChip® one-cycle target labelling and control reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
50µg/ml of labeled cRNA was hybridized at 45°C for 16h using the Affymetrix GeneChip hybridization oven. Washing and staining was performed on the Affymetrix Fluidics 450 using the EukGE-WS2v4 protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Data processing |
Data were processed with GCOS 1.4 (TGT = 150) and subsequently imported and further analyzed in the online database (www.bioretis.de)
|
| |
|
| Submission date |
Jul 04, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Biljana Smiljanovic |
| E-mail(s) |
biljana.smiljanovic@charite.de
|
| Phone |
+49 30 450 513 296
|
| Organization name |
Charité - Universitätsmedizin Berlin
|
| Department |
Department of Rheumatology and Clinical Immunology
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE100786 |
Expression data of bone marrow and blood monocytes from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) |
|
