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Sample GSM2688975 Query DataSets for GSM2688975
Status Public on Sep 22, 2017
Title Pml wt_apap6h_1
Sample type RNA
 
Source name Pml wt liver, 6h after apap ip
Organism Mus musculus
Characteristics gender: female
tissue: Liver
age (months): 2.5
strain: BALB/cByJ
Treatment protocol The mice were i.p. injected with Acetaminophen (Sigma) 300mg/kg solved in warmed PBS. They were sacrificed by cervical dislocation at indicated time. Untreated mice were sacrificed as a control. Liver samples are collected and stabilized in RNAlater solution and kept in -20. .
Growth protocol Pml wt and Pml KO mice with BALB/cByJ mice are maintained in a 12-hour light/dark cycle animal facility under SPF conditions, with free access to water and food.
Extracted molecule total RNA
Extraction protocol total RNA were extracted from using miRNeasy kit (Qiagen)
Label biotin
Label protocol 100ng of total RNA were amplified to generate cRNA; 10µg of cRNA were converted into single stranded DNA, based on the WT cDNA Synthesis Kit (Affymetrix). 5,5µg of cDNA were fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized onto GeneChip Mouse Gene 1.1 ST Arrays (Affymetrix) using the GeneTitan platform at the Genomics platform, Translational Resarch Dept, Institut Curie , Paris, France. 3.3µg of targets were hybridized according Affymetrix recommendations.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description gene expression data from mouse liver sample using Affymetrix Mouse Gene 1.1 ST
Data processing Raw data were processed with Expression Console from Affymetrix. A RMA normlisation was applied to control to data.
 
Submission date Jun 28, 2017
Last update date Jan 23, 2018
Contact name Michiko Kawakita
E-mail(s) rinko.kawa@gmail.com
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
 
Platform ID GPL11533
Series (2)
GSE100616 Gene expression profiling of Pml wt and Pml KO mice liver with acetaminophen (apap) overdose (300mg/kg) i.p.
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress

Data table header descriptions
ID_REF
VALUE gcRMA

Data table
ID_REF VALUE
10344614 93.65124
10344616 17.75471
10344620 73.11584
10344622 246.0477
10344624 1030.773
10344633 832.0673
10344637 1045.579
10344653 29.85748
10344658 573.5553
10344674 44.42663
10344679 32.74785
10344707 605.9418
10344713 6107.413
10344715 39.40119
10344717 181.2846
10344719 96.73028
10344721 13.75703
10344723 163.6651
10344725 1477.188
10344741 1017.206

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM2688975_EX35_ARN0005_s1h1_E05_mGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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