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Sample GSM2688974 Query DataSets for GSM2688974
Status Public on Sep 22, 2017
Title Pml KO_apap2h_2
Sample type RNA
Source name Pml KO liver, 2h after apap ip
Organism Mus musculus
Characteristics gender: female
tissue: Liver
age (months): 2.5
strain: BALB/cByJ
Treatment protocol The mice were i.p. injected with Acetaminophen (Sigma) 300mg/kg solved in warmed PBS. They were sacrificed by cervical dislocation at indicated time. Untreated mice were sacrificed as a control. Liver samples are collected and stabilized in RNAlater solution and kept in -20. .
Growth protocol Pml wt and Pml KO mice with BALB/cByJ mice are maintained in a 12-hour light/dark cycle animal facility under SPF conditions, with free access to water and food.
Extracted molecule total RNA
Extraction protocol total RNA were extracted from using miRNeasy kit (Qiagen)
Label biotin
Label protocol 100ng of total RNA were amplified to generate cRNA; 10µg of cRNA were converted into single stranded DNA, based on the WT cDNA Synthesis Kit (Affymetrix). 5,5µg of cDNA were fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized onto GeneChip Mouse Gene 1.1 ST Arrays (Affymetrix) using the GeneTitan platform at the Genomics platform, Translational Resarch Dept, Institut Curie , Paris, France. 3.3µg of targets were hybridized according Affymetrix recommendations.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description gene expression data from mouse liver sample using Affymetrix Mouse Gene 1.1 ST
Data processing Raw data were processed with Expression Console from Affymetrix. A RMA normlisation was applied to control to data.
Submission date Jun 28, 2017
Last update date Jan 23, 2018
Contact name Michiko Kawakita
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
Platform ID GPL11533
Series (2)
GSE100616 Gene expression profiling of Pml wt and Pml KO mice liver with acetaminophen (apap) overdose (300mg/kg) i.p.
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress

Data table header descriptions

Data table
10344614 58.52047
10344616 41.90321
10344620 77.19312
10344622 279.4326
10344624 1569.662
10344633 650.8453
10344637 714.0784
10344653 27.53534
10344658 859.5557
10344674 41.81836
10344679 38.15183
10344707 953.8721
10344713 7223.744
10344715 29.37166
10344717 157.6215
10344719 70.02321
10344721 20.83397
10344723 84.9327
10344725 2098.653
10344741 639.2548

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.

Supplementary file Size Download File type/resource
GSM2688974_EX35_ARN0010_s1h1_B07_mGene.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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