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Sample GSM2688973 Query DataSets for GSM2688973
Status Public on Sep 22, 2017
Title Pml KO_apap2h_1
Sample type RNA
 
Source name Pml KO liver, 2h after apap ip
Organism Mus musculus
Characteristics gender: female
tissue: Liver
age (months): 2.5
strain: BALB/cByJ
Treatment protocol The mice were i.p. injected with Acetaminophen (Sigma) 300mg/kg solved in warmed PBS. They were sacrificed by cervical dislocation at indicated time. Untreated mice were sacrificed as a control. Liver samples are collected and stabilized in RNAlater solution and kept in -20. .
Growth protocol Pml wt and Pml KO mice with BALB/cByJ mice are maintained in a 12-hour light/dark cycle animal facility under SPF conditions, with free access to water and food.
Extracted molecule total RNA
Extraction protocol total RNA were extracted from using miRNeasy kit (Qiagen)
Label biotin
Label protocol 100ng of total RNA were amplified to generate cRNA; 10µg of cRNA were converted into single stranded DNA, based on the WT cDNA Synthesis Kit (Affymetrix). 5,5µg of cDNA were fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized onto GeneChip Mouse Gene 1.1 ST Arrays (Affymetrix) using the GeneTitan platform at the Genomics platform, Translational Resarch Dept, Institut Curie , Paris, France. 3.3µg of targets were hybridized according Affymetrix recommendations.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description gene expression data from mouse liver sample using Affymetrix Mouse Gene 1.1 ST
Data processing Raw data were processed with Expression Console from Affymetrix. A RMA normlisation was applied to control to data.
 
Submission date Jun 28, 2017
Last update date Jan 23, 2018
Contact name Michiko Kawakita
E-mail(s) rinko.kawa@gmail.com
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
 
Platform ID GPL11533
Series (2)
GSE100616 Gene expression profiling of Pml wt and Pml KO mice liver with acetaminophen (apap) overdose (300mg/kg) i.p.
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress

Data table header descriptions
ID_REF
VALUE gcRMA

Data table
ID_REF VALUE
10344614 61.63118
10344616 34.30162
10344620 90.51511
10344622 297.8126
10344624 1509.892
10344633 758.6094
10344637 691.3926
10344653 28.22521
10344658 863.4827
10344674 44.91161
10344679 40.98919
10344707 930.9877
10344713 7383.875
10344715 31.44246
10344717 137.8261
10344719 69.18267
10344721 18.17126
10344723 167.2406
10344725 2037.672
10344741 809.6492

Total number of rows: 35556

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM2688973_EX35_ARN0009_s1h1_A07_mGene.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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