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Sample GSM2688965 Query DataSets for GSM2688965
Status Public on Sep 22, 2017
Title Pml KO_fasted 6h 2
Sample type RNA
Source name Pml KO liver, fasted 6h
Organism Mus musculus
Characteristics gender: female
tissue: liver
age (months): 3
strain: BALB/cByJ
Treatment protocol The food were removed. Free access to water. Six hours later, mice are sacrificed by cervical dislocation. Liver samples are collected and stabilized in RNAlater solution and kept in -20.
Growth protocol Pml wt and Pml KO mice with BALB/cByJ mice are maintained in a 12-hour light/dark cycle animal facility under SPF conditions, with free access to water and food.
Extracted molecule total RNA
Extraction protocol total RNA were extracted from using miRNeasy kit (Qiagen)
Label biotin
Label protocol 100ng of total RNA were amplified to generate cRNA; 10µg of cRNA were converted into single stranded DNA, based on the WT cDNA Synthesis Kit (Affymetrix). 5,5µg of cDNA were fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized onto GeneChip Mouse Gene 1.1 ST Arrays (Affymetrix) using the GeneTitan platform at the Genomics platform, Translational Resarch Dept, Institut Curie , Paris, France. 3.3µg of targets were hybridized according Affymetrix recommendations.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description gene expression data from mouse liver sample using Affymetrix Mouse Gene 2.1 ST
Data processing Raw data were processed with Expression Console from Affymetrix. A RMA normlisation was applied to control to data.
Submission date Jun 28, 2017
Last update date Jan 23, 2018
Contact name Michiko Kawakita
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
Platform ID GPL17400
Series (2)
GSE100615 Gene expression profiling of Pml wt and Pml KO mice liver after fasted 6h
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress

Data table header descriptions

Data table
17210850 8.062737
17210852 27.57366
17210855 1476.683
17210869 569.4319
17210883 24.3276
17210887 477.6133
17210904 17.00773
17210912 646.5433
17210947 49.35122
17210953 20.31931
17210984 1041.147
17210994 90.51679
17210996 24.12021
17210998 8.530072
17211000 53.47131
17211004 1765.141
17211023 60.78706
17211033 61.98133
17211043 482.2547
17211066 23.28996

Total number of rows: 41345

Table truncated, full table size 723 Kbytes.

Supplementary file Size Download File type/resource
GSM2688965_EX35_ARN0041_s1h1_A07_mGene2.1.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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