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Sample GSM2688964 Query DataSets for GSM2688964
Status Public on Sep 22, 2017
Title Pml KO_fasted 6h 1
Sample type RNA
Source name Pml KO liver, fasted 6h
Organism Mus musculus
Characteristics gender: female
tissue: liver
age (months): 3
strain: BALB/cByJ
Treatment protocol The food were removed. Free access to water. Six hours later, mice are sacrificed by cervical dislocation. Liver samples are collected and stabilized in RNAlater solution and kept in -20.
Growth protocol Pml wt and Pml KO mice with BALB/cByJ mice are maintained in a 12-hour light/dark cycle animal facility under SPF conditions, with free access to water and food.
Extracted molecule total RNA
Extraction protocol total RNA were extracted from using miRNeasy kit (Qiagen)
Label biotin
Label protocol 100ng of total RNA were amplified to generate cRNA; 10µg of cRNA were converted into single stranded DNA, based on the WT cDNA Synthesis Kit (Affymetrix). 5,5µg of cDNA were fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized onto GeneChip Mouse Gene 1.1 ST Arrays (Affymetrix) using the GeneTitan platform at the Genomics platform, Translational Resarch Dept, Institut Curie , Paris, France. 3.3µg of targets were hybridized according Affymetrix recommendations.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description gene expression data from mouse liver sample using Affymetrix Mouse Gene 2.1 ST
Data processing Raw data were processed with Expression Console from Affymetrix. A RMA normlisation was applied to control to data.
Submission date Jun 28, 2017
Last update date Jan 23, 2018
Contact name Michiko Kawakita
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
Platform ID GPL17400
Series (2)
GSE100615 Gene expression profiling of Pml wt and Pml KO mice liver after fasted 6h
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress

Data table header descriptions

Data table
17210850 16.93011
17210852 23.98014
17210855 1591.155
17210869 569.1874
17210883 23.91178
17210887 441.6112
17210904 17.4754
17210912 507.4385
17210947 47.30323
17210953 14.64141
17210984 919.5807
17210994 98.38649
17210996 13.39872
17210998 7.576958
17211000 47.83722
17211004 1914.478
17211023 45.09265
17211033 72.79437
17211043 562.5838
17211066 28.29565

Total number of rows: 41345

Table truncated, full table size 723 Kbytes.

Supplementary file Size Download File type/resource
GSM2688964_EX35_ARN0040_s1h1_H05_mGene2.1.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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