GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM2688963 Query DataSets for GSM2688963
Status Public on Sep 22, 2017
Title Pml wt_fasted 6h 3
Sample type RNA
Source name Pml wt liver, fasted 6h
Organism Mus musculus
Characteristics gender: female
tissue: liver
age (months): 3
strain: BALB/cByJ
Treatment protocol The food were removed. Free access to water. Six hours later, mice are sacrificed by cervical dislocation. Liver samples are collected and stabilized in RNAlater solution and kept in -20.
Growth protocol Pml wt and Pml KO mice with BALB/cByJ mice are maintained in a 12-hour light/dark cycle animal facility under SPF conditions, with free access to water and food.
Extracted molecule total RNA
Extraction protocol total RNA were extracted from using miRNeasy kit (Qiagen)
Label biotin
Label protocol 100ng of total RNA were amplified to generate cRNA; 10µg of cRNA were converted into single stranded DNA, based on the WT cDNA Synthesis Kit (Affymetrix). 5,5µg of cDNA were fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized onto GeneChip Mouse Gene 1.1 ST Arrays (Affymetrix) using the GeneTitan platform at the Genomics platform, Translational Resarch Dept, Institut Curie , Paris, France. 3.3µg of targets were hybridized according Affymetrix recommendations.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description gene expression data from mouse liver sample using Affymetrix Mouse Gene 2.1 ST
Data processing Raw data were processed with Expression Console from Affymetrix. A RMA normlisation was applied to control to data.
Submission date Jun 28, 2017
Last update date Jan 23, 2018
Contact name Michiko Kawakita
Organization name INSERM
Department U944
Street address 1 av. Claude-Vellefaux
City Paris
ZIP/Postal code 75010
Country France
Platform ID GPL17400
Series (2)
GSE100615 Gene expression profiling of Pml wt and Pml KO mice liver after fasted 6h
GSE100617 PML is a ROS sensor activating p53 upon oxidative stress

Data table header descriptions

Data table
17210850 9.726678
17210852 26.30497
17210855 1400.602
17210869 604.7562
17210883 23.93994
17210887 525.2411
17210904 23.8948
17210912 748.7178
17210947 44.47517
17210953 19.57675
17210984 913.7172
17210994 85.17136
17210996 19.44967
17210998 10.31816
17211000 60.74537
17211004 1342.816
17211023 49.36428
17211033 51.60683
17211043 475.6791
17211066 20.9651

Total number of rows: 41345

Table truncated, full table size 723 Kbytes.

Supplementary file Size Download File type/resource
GSM2688963_EX35_ARN0039_s1h1_G05_mGene2.1.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap