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Sample GSM2671898 Query DataSets for GSM2671898
Status Public on Mar 10, 2020
Title Myb_mutant_biol_rep2_tech_rep4
Sample type RNA
 
Source name Wing discs of Myb mutant 3rd instar larvae
Organism Drosophila melanogaster
Characteristics genotype: Myb^MH107: mutation induced by P-element mobilization by Delta2-3 transposase
phenotype: Lethal-early pupal stage
gender: Male
developmental stage: 3rd instar larvae
tissue: Imaginal wing disc
Treatment protocol 3rd instar larvae were dissected in 1X PBS (pH 7.4)
Growth protocol Flies were mantined at 25°C in malt-based media (Archon Scientific).
Extracted molecule total RNA
Extraction protocol RNA was extracted using TRIzol (Invitrogen) and purified with RNeasy Mini Kit (Qiagen). Double-stranded cDNA was generated using Double-Stranded cDNA Synthesis Kit with random hexamer (Invitrogen). RNA and cDNA quality were analyzed using Experion RNA and DNA analysis kits (Bio-Rad), respectively.
Label Cy3
Label protocol Labeling was performed following their standard protocol. See www.roche.com.
 
Hybridization protocol Hybridization was performed following their standard protocol. See www.roche.com.
Scan protocol Scanning was performed following their standard protocol. See www.roche.com.
Description Myb mutant imaginal wing discs of 3rd instar larvae. Fourth technical replicate of the second biological replicate
Data processing Raw data (.pair files) were normalized in ArrayStar software version 12.0.0 (DNASTAR, Inc, Madison, WI). The robust multichip analysis (RMA) algorithm was used for background correction, quantile normalization, and median polish summarization. A Student’s t test corrected for multiple testing with the Benjamini and Hochberg false discovery rate (FDR) method was calculated for each experiment.
 
Submission date Jun 16, 2017
Last update date Mar 11, 2020
Contact name David H Price
E-mail(s) david-price@uiowa.edu
Organization name University of Iowa
Department Biochemistry and Molecular Biology
Lab 260 EMRB
Street address 500 Newton Road
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL15352
Series (2)
GSE100142 Gene expression profiling of Drosophila melangoaster Myb mutant wing discs of 3rd instar larvae. [NimbleGenGeneExpression-12plex]
GSE100143 Drosophila melangoaster Myb mutant wing discs of 3rd instar larvae.

Data table header descriptions
ID_REF
VALUE Gene mean signal intensity after normalization.

Data table
ID_REF VALUE
FBtr0299512 14.158
FBtr0299510 14.158
FBtr0112743 14.158
FBtr0073595 14.158
FBtr0088263 14.158
FBtr0100026 14.158
FBtr0072031 14.158
FBtr0100333 14.158
FBtr0100332 14.158
FBtr0083262 14.158
FBtr0113259 14.158
FBtr0091473 14.158
FBtr0091472 14.158
FBtr0091471 14.158
FBtr0112577 14.158
FBtr0111171 14.158
FBtr0111172 14.158
FBtr0100157 14.158
FBtr0100156 14.158
FBtr0078750 14.158

Total number of rows: 21051

Table truncated, full table size 410 Kbytes.




Supplementary file Size Download File type/resource
GSM2671898_myb_5_A01_2_555577_1_532.pair.gz 2.2 Mb (ftp)(http) PAIR
Processed data are available on Series record

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