|
| Status |
Public on Mar 10, 2020 |
| Title |
Myb_mutant_biol_rep2_tech_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Wing discs of Myb mutant 3rd instar larvae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: Myb^MH107: mutation induced by P-element mobilization by Delta2-3 transposase
phenotype: Lethal-early pupal stage
gender: Male
developmental stage: 3rd instar larvae
tissue: Imaginal wing disc
|
| Treatment protocol |
3rd instar larvae were dissected in 1X PBS (pH 7.4)
|
| Growth protocol |
Flies were mantined at 25°C in malt-based media (Archon Scientific).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIzol (Invitrogen) and purified with RNeasy Mini Kit (Qiagen). Double-stranded cDNA was generated using Double-Stranded cDNA Synthesis Kit with random hexamer (Invitrogen). RNA and cDNA quality were analyzed using Experion RNA and DNA analysis kits (Bio-Rad), respectively.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed following their standard protocol. See www.roche.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed following their standard protocol. See www.roche.com.
|
| Scan protocol |
Scanning was performed following their standard protocol. See www.roche.com.
|
| Description |
Myb mutant imaginal wing discs of 3rd instar larvae. Third technical replicate of the second biological replicate
|
| Data processing |
Raw data (.pair files) were normalized in ArrayStar software version 12.0.0 (DNASTAR, Inc, Madison, WI). The robust multichip analysis (RMA) algorithm was used for background correction, quantile normalization, and median polish summarization. A Student’s t test corrected for multiple testing with the Benjamini and Hochberg false discovery rate (FDR) method was calculated for each experiment.
|
| |
|
| Submission date |
Jun 16, 2017 |
| Last update date |
Mar 11, 2020 |
| Contact name |
David H Price |
| E-mail(s) |
david-price@uiowa.edu
|
| Organization name |
University of Iowa
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
260 EMRB
|
| Street address |
500 Newton Road
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL15352 |
| Series (2) |
| GSE100142 |
Gene expression profiling of Drosophila melangoaster Myb mutant wing discs of 3rd instar larvae. [NimbleGenGeneExpression-12plex] |
| GSE100143 |
Drosophila melangoaster Myb mutant wing discs of 3rd instar larvae. |
|
