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Sample GSM2636226 Query DataSets for GSM2636226
Status Public on Jun 25, 2018
Title OSE7 with miR-200 cluster 1 and 2 overexpressed
Sample type RNA
 
Source name Normal ovarian surface epithelial cells OSE7 with over-expression of miR-200 cluster 1 and 2 (miR-200a, miR-200b, miR-200c, miR-141 and miR-429) grown in Matrigel for 7 days
Organism Homo sapiens
Characteristics tissue: Ovarian surface epithelial cells
genotype: Normal
age: immortalized
Treatment protocol The day for collecion, culture medium were removed and the cysts formed were rinsed with PBS and Trizol solution (Ambion) was added to the culture.
Growth protocol Eight-well glass-chambered slides (BD Biosciences) were coated with growth factor-reduced Matrigel matrix without phenol red (BD Biosciences) . Primary cultures of normal OSE and cancer cells were trypsinized and resuspended as single cells in complete medium with 10% FBS and 2% Matrigel. Fifty thousand single cells were seeded into each Matrigel-coated well. Complete medium with 2% Matrigel was replaced every 4 days. 3D structures would be formed after 4 to 7 days after seeding.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions with subsequent purification by chloroform extraction and isopropanol precipitation
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (GeneChip WT Terminal Labling User Manual, 2009-2010, Affymetrix).
 
Hybridization protocol Following fragmentation, 3.5 ug of cRNA were hybridized for 48C on GeneChip Gene 1.1 ST Array in GeneAtlas Hybridization Station. GeneChip was washed and stained according to GeneAtlas TM System User’s Guide (P/N 08-0246)
Scan protocol GeneChips were scanned using the GeneAtlas Instrument.
Description Gene expression data from OSE7 with over-expression of miR-200 cluster 1 and 2 (miR-200a, miR-200b, miR-200c, miR-141 and miR-429) grown in Matrigel for 7 days
Data processing All gene expression data was normalized and summarized with Robust Multi-array Average method RMA algorithm with with Affymetrix Power Tools package using Affymetrix default analysis settings.
 
Submission date May 23, 2017
Last update date Jun 25, 2018
Contact name pui wah Choi
E-mail(s) puiwahchoi@gmail.com
Phone 6173563166
Organization name Brigham and Women's Hospital
Department OB/GYB
Lab GYN oncology, Alex Ng
Street address 221 longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL11532
Series (1)
GSE99217 Expression data from ovarian cells growing in three dimensional culture (Matrigel) with altered expression of miR-200s

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
7896738 3.089045
7896740 2.985917
7896742 7.505321
7896744 3.69932
7896746 7.241972
7896748 8.273165
7896750 7.540502
7896752 9.793321
7896754 5.602909
7896756 4.317486
7896759 4.710135
7896761 4.769217
7896779 5.686697
7896798 4.829937
7896817 6.342953
7896822 7.769051
7896859 5.466795
7896861 3.818108
7896863 4.610094
7896865 5.425064

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM2636226_OSE7-KI1KI2.cel.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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