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Sample GSM2636225 Query DataSets for GSM2636225
Status Public on Jun 25, 2018
Title OSE7 with miR-200 cluster 2 overexpressed
Sample type RNA
Source name Normal ovarian surface epithelial cells with over-expression of miR-200 cluster 2 (miR-200c, miR-141) grown in Matrigel for 7 days
Organism Homo sapiens
Characteristics tissue: Ovarian surface epithelial cells
genotype: Normal
age: immortalized
Treatment protocol The day for collecion, culture medium were removed and the cysts formed were rinsed with PBS and Trizol solution (Ambion) was added to the culture.
Growth protocol Eight-well glass-chambered slides (BD Biosciences) were coated with growth factor-reduced Matrigel matrix without phenol red (BD Biosciences) . Primary cultures of normal OSE and cancer cells were trypsinized and resuspended as single cells in complete medium with 10% FBS and 2% Matrigel. Fifty thousand single cells were seeded into each Matrigel-coated well. Complete medium with 2% Matrigel was replaced every 4 days. 3D structures would be formed after 4 to 7 days after seeding.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions with subsequent purification by chloroform extraction and isopropanol precipitation
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA (GeneChip WT Terminal Labling User Manual, 2009-2010, Affymetrix).
Hybridization protocol Following fragmentation, 3.5 ug of cRNA were hybridized for 48C on GeneChip Gene 1.1 ST Array in GeneAtlas Hybridization Station. GeneChip was washed and stained according to GeneAtlas TM System User’s Guide (P/N 08-0246)
Scan protocol GeneChips were scanned using the GeneAtlas Instrument.
Description Gene expression data from OSE7 with over-expression of miR-200 cluster 2 (miR-200c, miR-141) grown in Matrigel for 7 days
Data processing All gene expression data was normalized and summarized with Robust Multi-array Average method RMA algorithm with with Affymetrix Power Tools package using Affymetrix default analysis settings.
Submission date May 23, 2017
Last update date Jun 25, 2018
Contact name pui wah Choi
Phone 6173563166
Organization name Brigham and Women's Hospital
Department OB/GYB
Lab GYN oncology, Alex Ng
Street address 221 longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL11532
Series (1)
GSE99217 Expression data from ovarian cells growing in three dimensional culture (Matrigel) with altered expression of miR-200s

Data table header descriptions

Data table
7896738 3.60493
7896740 3.399707
7896742 7.639593
7896744 4.917272
7896746 8.133341
7896748 9.938467
7896750 9.203506
7896752 11.08677
7896754 5.420014
7896756 4.549754
7896759 4.382804
7896761 4.994929
7896779 5.542185
7896798 4.302899
7896817 5.896357
7896822 7.63974
7896859 4.481622
7896861 3.917355
7896863 4.274202
7896865 5.178372

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.

Supplementary file Size Download File type/resource
GSM2636225_OSE7-KI2.cel.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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