NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2600157 Query DataSets for GSM2600157
Status Public on Apr 10, 2018
Title Control subject 6045 blood collection 03 [D9]
Sample type RNA
 
Source name blood
Organism Homo sapiens
Characteristics subject id: s6045
fatigue_category: unknown
subject group: Control
study phase: baseline
timepoint: t03
Treatment protocol Blood was drawn every 4 hours during time awake on day 2 (Baseline), day 4 (Experimental), and day 6 (Recovery) of the 7 days in the sleep lab, amounting to 4 draws per day for 12 total timepoints per subject. On all 7 days, Control (C) subjects had 10 h TIB. The Sleep Deprived (SD) subjects experienced 10 h TIB during Baseline and Recovery days, but underwent 62 h continued wakefulness in the Experimental phase. Blood draws on day 4 (Experimental) for the Sleep Deprived subjects correspond to hours 24-36 of continued wakefulness.
Growth protocol Blood was collected in PAXgene tubes from healthy adult human volunteers.
Extracted molecule total RNA
Extraction protocol RNA was extracted from blood collected in PAXgene™ tubes using a QIAcube robotic workstation (Qiagen).
Label biotin
Label protocol Reverse transcription was performed with Ovation Pico WTA System V2 kit (NuGEN), and fragmentation and biotin labeling was performed using the Encore® Biotin module (NuGEN).
 
Hybridization protocol Hybridization cocktail was prepared according to the NuGEN Encore® Biotin module manual. Affymetrix GeneChip Human Gene 1.0 ST arrays were prewet with hybridization solution, and then incubated with hybridization cocktail.
Scan protocol Affymetrix Wash and Stain kit reagents and fluidics protocol were used with the GeneChip Fluidics Station 450 for post-hybridization chip processing. Scanning was done on an Affymetrix Scanner with G7 upgrade.
Description C_6045_t03
Reanalysis of sample C_6045_t03 in GSE98564
Data processing Transcript Cluster-level expression data was derived with the RMA algorithm in the R/oligo package v. 1.32.0. Data were filtered for low expression, only retaining a Transcript Cluster if at least 6 samples had log2 expression >6. This Series contains values for all cel files in GSE98564 except those for the 3 fatigue resistant subjects.
 
Submission date May 04, 2017
Last update date Apr 10, 2018
Contact name Hilary A Uyhelji
Organization name Federal Aviation Administration
Department Civil Aerospace Medical Institute
Street address 6500 S. MacArthur Blvd.
City Oklahoma City
State/province OK
ZIP/Postal code 73169
Country USA
 
Platform ID GPL6244
Series (2)
GSE98566 Gene expression biomarkers for neurobehavioral impairment from total sleep deprivation microarray data [D9]
GSE98582 Exploring gene expression biomarker candidates for neurobehavioral impairment from total sleep deprivation

Data table header descriptions
ID_REF
VALUE RMA log2 expression

Data table
ID_REF VALUE
7896742 6.957142804
7896746 8.899791908
7896748 6.413431429
7896750 4.750609668
7896752 9.067820471
7896817 8.427961718
7896878 5.824203682
7897006 5.26126188
7897068 6.756319051
7897078 8.216162956
7897119 6.069212374
7897172 7.537215897
7897236 6.209862696
7897288 5.220299448
7897305 5.890572064
7897370 7.916052057
7897404 7.724121884
7897482 7.02961822
7897522 7.922081459
7897527 7.086559225

Total number of rows: 8515

Table truncated, full table size 165 Kbytes.




Supplementary file Size Download File type/resource
GSM2600157_6045t3_071012_HuGene-1_0-st-v1_.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap