|
| Status |
Public on Apr 22, 2017 |
| Title |
Vehicle-treated T cells [DA_13_04.RCC] |
| Sample type |
RNA |
| |
|
| Source name |
T cells
|
| Organism |
Mus musculus |
| Characteristics |
agent: Vehicle
tissue: Lung tumor
strain: Mixed background
gemm model: KrasTrp53
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy RNA Isolation Kit utilized to extract RNA from purified T cells and macrophages within tumor cell suspensions
|
| Label |
multiple fluorophores
|
| Label protocol |
Nanostring
|
| |
|
| Hybridization protocol |
nCounter analysis system
|
| Scan protocol |
nCounter analysis system
|
| Data processing |
nSolver 2.6 software with HumanPanCancerImmunology_1.0.36 analysis module. Individual samples analyzed with different codesets were processed according to standard Nanostring normalization recommendations. In short, the background subtraction step was skipped and housekeeping genes with the counts below 70 were removed from the list. Seven genes with the lowest percent of coefficient of variation were selected for normalization.
|
| |
|
| Submission date |
Apr 21, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Ruben Dries |
| E-mail(s) |
rubendries@gmail.com
|
| Organization name |
DFCI
|
| Department |
Computational Biology
|
| Lab |
Longwood Center
|
| Street address |
360 Longwood Ave
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL22364 |
| Series (2) |
| GSE98044 |
Single-cell profiling of tumor infiltrating T cells and macrophages [Nanostring] |
| GSE98049 |
Synergistic Immunostimulatory Effects and Therapeutic Benefit of Combined Histone Deacetylase and Bromodomain Inhibition in Non-small Cell Lung Cancer. |
|
