|
| Status |
Public on Dec 26, 2018 |
| Title |
Primate fetal hepatic response to maternal obesity [5304687013_A] |
| Sample type |
RNA |
| |
|
| Source name |
Baboon fetal liver, maternal obese
|
| Organism |
Papio hamadryas |
| Characteristics |
condition: maternal obese
gender: male
age: 165 days or 0.9 gestation
tissue: fetal liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from fetal livers using TRI Reagent (Ambion, USA) according to the manufacturer's instructions, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA integrity and concentration were determined by an Agilent 2100 Bioanalyzer and UV-Vis Spectrophotometer (NanoDrop 2000).
|
| Label |
Biotin
|
| Label protocol |
Complementary RNA (cRNA) was synthesized from total RNA using the Illumina Total Prep 96 RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Sample name: 5304687013_A
|
| Data processing |
Gene expression data were processed and analyzed using GenomeStudio (Illumina) and GeneSifter (PerkinElmer). Gene array data were median normalized and log2 transformed (GeneSifter). Cluster analysis of gene expression was done using hierarchical clustering by adjusting expression values to a mean of zero and scale to standard deviation of one in Partek® Genomics Suite®. Supplementary Table 1 contains differentially expressed genes between MO and CON fetal livers at 0.9G.
|
| |
|
| Submission date |
Apr 09, 2017 |
| Last update date |
Dec 26, 2018 |
| Contact name |
Sobha Puppala |
| E-mail(s) |
sobhap@icloud.com
|
| Phone |
210-258-9770
|
| Organization name |
Texas Biomedical Research Institute
|
| Department |
Genetics
|
| Street address |
7620 NW Loop 410
|
| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78227 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE97554 |
Primate fetal hepatic response to maternal obesity: epigenetic signaling pathways and lipid accumulation [gene expression] |
| GSE99718 |
Primate fetal hepatic response to maternal obesity: epigenetic signaling pathways and lipid accumulation |
|
