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Sample GSM2572023 Query DataSets for GSM2572023
Status Public on Dec 26, 2018
Title Primate fetal hepatic response to maternal obesity [5304687008_E]
Sample type RNA
 
Source name Baboon fetal liver, control
Organism Papio hamadryas
Characteristics condition: control
gender: female
age: 165 days or 0.9 gestation
tissue: fetal liver
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from fetal livers using TRI Reagent (Ambion, USA) according to the manufacturer's instructions, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA integrity and concentration were determined by an Agilent 2100 Bioanalyzer and UV-Vis Spectrophotometer (NanoDrop 2000).
Label Biotin
Label protocol Complementary RNA (cRNA) was synthesized from total RNA using the Illumina Total Prep 96 RNA Amplification Kit.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description Sample name: 5304687008_E
Data processing Gene expression data were processed and analyzed using GenomeStudio (Illumina) and GeneSifter (PerkinElmer). Gene array data were median normalized and log2 transformed (GeneSifter). Cluster analysis of gene expression was done using hierarchical clustering by adjusting expression values to a mean of zero and scale to standard deviation of one in PartekĀ® Genomics SuiteĀ®. Supplementary Table 1 contains differentially expressed genes between MO and CON fetal livers at 0.9G.
 
Submission date Apr 09, 2017
Last update date Dec 26, 2018
Contact name Sobha Puppala
E-mail(s) sobhap@icloud.com
Phone 210-258-9770
Organization name Texas Biomedical Research Institute
Department Genetics
Street address 7620 NW Loop 410
City San Antonio
State/province TX
ZIP/Postal code 78227
Country USA
 
Platform ID GPL6947
Series (2)
GSE97554 Primate fetal hepatic response to maternal obesity: epigenetic signaling pathways and lipid accumulation [gene expression]
GSE99718 Primate fetal hepatic response to maternal obesity: epigenetic signaling pathways and lipid accumulation

Data table header descriptions
ID_REF
VALUE Median normalized and log2 transformed data
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.926641607 0.5349144
ILMN_2055271 6.694034659 0.01449275
ILMN_1736007 9.727389384 0
ILMN_2383229 11.54153807 0
ILMN_1806310 12.1819821 0
ILMN_1779670 6.059204701 0.2490119
ILMN_1653355 6.240380928 0.0974967
ILMN_1717783 5.77211316 0.8853755
ILMN_1705025 6.101300032 0.1949934
ILMN_1814316 6.078730012 0.2252964
ILMN_2359168 6.114466977 0.1818182
ILMN_1731507 5.860957833 0.6969697
ILMN_1787689 6.081866863 0.2226614
ILMN_1745607 8.594151395 0
ILMN_2136495 5.75437472 0.9090909
ILMN_1668111 5.760999047 0.8998682
ILMN_2295559 5.849308011 0.7299078
ILMN_1735045 6.169746657 0.1383399
ILMN_1680754 6.062520246 0.2463768
ILMN_2375184 6.007591784 0.3386034

Total number of rows: 48803

Table truncated, full table size 1637 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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