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Sample GSM2561518 Query DataSets for GSM2561518
Status Public on Apr 01, 2019
Title Plaster cast_skeletal muscle
Sample type RNA
Source name Immobilization plaster cast (11days)
Organism Mus musculus
Characteristics strain: C57BL/6
age: 9 weeks
gender: male
treated with: a plaster cast for 11 days
tissue: skeletal muscle
Extracted molecule total RNA
Extraction protocol RNA was isolated from the gastrocnemius skeletal muscle of control mice and plaster cast mice. RNA was purified using the RNeasy Mini kit (Qiagen, Hilden, Germany).
Label Cy3
Label protocol Cy3-labeled cRNA (1.65 μg) was mixed and fragmented according to the Agilent Technologies protocol.
Hybridization protocol cRNA was prepared according to the standard Agilent protocol. Agilent platform Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) was used.
Scan protocol Arrays were scanned on an Agilent Microarray Scanner to detect Cy3 fluorescence.
Data processing The scanned images were analyzed using Feature Extraction Software 9.5 (Agilent) with default parameters (protocol: GE1-v5_95_Feb07 and Grid: 026655_D_F_20100123 to obtain background-subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction Software as Non-uniform outliers were excluded.
Submission date Apr 02, 2017
Last update date Apr 01, 2019
Contact name Yukino Hatazawa
Organization name Kyoto Prefectural University
Street address 1-5 Hangi-cho, Shimogamo, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8522
Country Japan
Platform ID GPL10333
Series (2)
GSE97312 Microarray analysis of mouse skeletal muscle atrophied by a plaster cast
GSE97315 Microarray analysis of atrophied skeletal muscle models

Data table header descriptions
VALUE Normalized signal intensity

Data table
1 1251
2 1
3 1
4 1
5 1
6 1
7 1
8 1
9 1
10 1
11 1
12 9
13 1
14 873
15 1
16 416
17 596
18 1
19 1
20 85

Total number of rows: 44397

Table truncated, full table size 549 Kbytes.

Supplementary file Size Download File type/resource
GSM2561518_US45102915_252665511989_S01_GE1-v5_95_Feb07_1_4.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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