|
| Status |
Public on Apr 01, 2019 |
| Title |
Plaster cast_skeletal muscle |
| Sample type |
RNA |
| |
|
| Source name |
Immobilization plaster cast (11days)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 9 weeks
gender: male
treated with: a plaster cast for 11 days
tissue: skeletal muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from the gastrocnemius skeletal muscle of control mice and plaster cast mice. RNA was purified using the RNeasy Mini kit (Qiagen, Hilden, Germany).
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA (1.65 μg) was mixed and fragmented according to the Agilent Technologies protocol.
|
| |
|
| Hybridization protocol |
cRNA was prepared according to the standard Agilent protocol. Agilent platform Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) was used.
|
| Scan protocol |
Arrays were scanned on an Agilent Microarray Scanner to detect Cy3 fluorescence.
|
| Data processing |
The scanned images were analyzed using Feature Extraction Software 9.5 (Agilent) with default parameters (protocol: GE1-v5_95_Feb07 and Grid: 026655_D_F_20100123 to obtain background-subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction Software as Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Apr 02, 2017 |
| Last update date |
Apr 01, 2019 |
| Contact name |
Yukino Hatazawa |
| Organization name |
Kyoto Prefectural University
|
| Street address |
1-5 Hangi-cho, Shimogamo, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8522 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10333 |
| Series (2) |
| GSE97312 |
Microarray analysis of mouse skeletal muscle atrophied by a plaster cast |
| GSE97315 |
Microarray analysis of atrophied skeletal muscle models |
|
