NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2552073 Query DataSets for GSM2552073
Status Public on Mar 30, 2017
Title WT_Rep1
Sample type SRA
 
Source name WT
Organism Drosophila melanogaster
Characteristics genotype: wild type
gender: male
age: 3 days old
tissue: adult testes
Growth protocol The flies were kept at 23°C
Extracted molecule polyA RNA
Extraction protocol 50 adult testes were dissected in PBS. Total RNA was isolated from testes, using TRIZol (Invitrogen) reagent, according to the manufacturer’s instructions.
One microgram of total RNA was processed for library preparation using the RNA TruSeq kit. The libraries were sequenced on the Illumina MiSeq system.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
 
Data processing Reads were aligned on D.melanogaster BDGP R6/dm6 genome assembly (https://genome-euro.ucsc.edu/) using TopHat (–r 200 –mate-std-dev 50) .
Transcript assembly was performed by Cufflinks (BDGP R6/dm6).
Genome_build: BDGP R6/dm6
Supplementary_files_format_and_content: gene abundance
 
Submission date Mar 28, 2017
Last update date May 15, 2019
Contact name Petr Laktionov
E-mail(s) laktionov@mcb.nsc.ru
Organization name Institute of molecular and cellular biology SD RAS
Lab Genomics lab
Street address Lavrenitev ave 8/2
City Novosibirsk
ZIP/Postal code 630090
Country Russia
 
Platform ID GPL16479
Series (1)
GSE97130 Genome-wide analysis of Su(var)3-9 distribution in chromosomes of Drosophila melanogaster [RNA-Seq_SuVar]
Relations
Reanalyzed by GSM3283157
BioSample SAMN06647900
SRA SRX2677262

Supplementary file Size Download File type/resource
GSM2552073_WT_Rep1_Cufflinks_gene_expression_BDGP6.tabular.txt.gz 533.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap