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Sample GSM2546992 Query DataSets for GSM2546992
Status Public on Jul 17, 2017
Title Left testis, short sperm line male 2011.M.548
Sample type RNA
 
Source name Zebra finch left testis, short sperm line male 2011.M.548
Organism Taeniopygia guttata
Characteristics tissue: testis
gender: male
age: adult
Treatment protocol Testes were treated with RNAlater and stored at -80C until RNA extraction
Growth protocol Male zebra finches were euthanized and dissected. Testes were extracted from both sides and washed with PBS.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA were hybridized on Zebra finch Gene 1.1 ST Array according to the manufacturer's instructions.
Scan protocol GeneChips were scanned using the Affymetrix GeneTitan.
Description Gene expression data from left testis of a short sperm line male
Data processing The data were analyzed with Affymetrix Expression Console ver 1.3 using default analysis settings and RMA (robust multi-array average) as normalization method.
 
Submission date Mar 23, 2017
Last update date Jul 17, 2017
Contact name Kang-Wook Kim
Organization name The University of Sheffield
Department Animal and Plant Sciences
Street address Western Bank
City Sheffield
ZIP/Postal code S10 2TN
Country United Kingdom
 
Platform ID GPL23222
Series (1)
GSE96970 Expression data from testes of zebra finch males artificially selected for long or short sperm

Data table header descriptions
ID_REF
VALUE Normalized processed intensity values in Log2 scale

Data table
ID_REF VALUE
15599166 5.02471
15599172 9.343118
15599176 8.677109
15599211 8.915052
15599226 8.831125
15599236 4.701111
15599247 8.692979
15599273 8.084727
15599278 9.155237
15599283 8.587523
15599294 4.563036
15599296 7.857165
15599301 8.088812
15599309 4.814532
15599314 5.906978
15599316 5.728869
15599319 12.33267
15599321 5.133343
15599331 7.603134
15599341 5.777147

Total number of rows: 23713

Table truncated, full table size 414 Kbytes.




Supplementary file Size Download File type/resource
GSM2546992_SL_2011.M.548.CEL.gz 5.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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