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Status |
Public on Jul 17, 2017 |
Title |
Left testis, short sperm line male 2011.M.548 |
Sample type |
RNA |
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Source name |
Zebra finch left testis, short sperm line male 2011.M.548
|
Organism |
Taeniopygia guttata |
Characteristics |
tissue: testis gender: male age: adult
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Treatment protocol |
Testes were treated with RNAlater and stored at -80C until RNA extraction
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Growth protocol |
Male zebra finches were euthanized and dissected. Testes were extracted from both sides and washed with PBS.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, cRNA were hybridized on Zebra finch Gene 1.1 ST Array according to the manufacturer's instructions.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan.
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Description |
Gene expression data from left testis of a short sperm line male
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Data processing |
The data were analyzed with Affymetrix Expression Console ver 1.3 using default analysis settings and RMA (robust multi-array average) as normalization method.
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Submission date |
Mar 23, 2017 |
Last update date |
Jul 17, 2017 |
Contact name |
Kang-Wook Kim |
Organization name |
The University of Sheffield
|
Department |
Animal and Plant Sciences
|
Street address |
Western Bank
|
City |
Sheffield |
ZIP/Postal code |
S10 2TN |
Country |
United Kingdom |
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|
Platform ID |
GPL23222 |
Series (1) |
GSE96970 |
Expression data from testes of zebra finch males artificially selected for long or short sperm |
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