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Sample GSM2546951 Query DataSets for GSM2546951
Status Public on Jul 17, 2017
Title Left testis, long sperm line male 2011.M.417
Sample type RNA
 
Source name Zebra finch left testis, long sperm line male 2011.M.417
Organism Taeniopygia guttata
Characteristics tissue: testis
gender: male
age: adult
Treatment protocol Testes were treated with RNAlater and stored at -80C until RNA extraction
Growth protocol Male zebra finches were euthanized and dissected. Testes were extracted from both sides and washed with PBS.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA were hybridized on Zebra finch Gene 1.1 ST Array according to the manufacturer's instructions.
Scan protocol GeneChips were scanned using the Affymetrix GeneTitan.
Description Gene expression data from left testis of a long sperm line male
Data processing The data were analyzed with Affymetrix Expression Console ver 1.3 using default analysis settings and RMA (robust multi-array average) as normalization method.
 
Submission date Mar 23, 2017
Last update date Jul 17, 2017
Contact name Kang-Wook Kim
Organization name The University of Sheffield
Department Animal and Plant Sciences
Street address Western Bank
City Sheffield
ZIP/Postal code S10 2TN
Country United Kingdom
 
Platform ID GPL23222
Series (1)
GSE96970 Expression data from testes of zebra finch males artificially selected for long or short sperm

Data table header descriptions
ID_REF
VALUE Normalized processed intensity values in Log2 scale

Data table
ID_REF VALUE
15599166 5.642801
15599172 8.476832
15599176 8.147988
15599211 9.010942
15599226 9.012211
15599236 5.474364
15599247 8.484391
15599273 8.401326
15599278 9.19118
15599283 8.695622
15599294 5.359581
15599296 7.635009
15599301 7.16997
15599309 5.478475
15599314 6.207964
15599316 6.997724
15599319 11.87555
15599321 6.351889
15599331 7.247031
15599341 6.250272

Total number of rows: 23713

Table truncated, full table size 414 Kbytes.




Supplementary file Size Download File type/resource
GSM2546951_LL_2011.M.417.CEL.gz 5.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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