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Sample GSM2537698 Query DataSets for GSM2537698
Status Public on Nov 11, 2017
Title 3Ib-CD-Active-Sigmoid-20
Sample type RNA
 
Source name Intestinal mucosa pinch biopsy
Organism Homo sapiens
Characteristics disease (uc = ulcerative colitis; cd = crohn's disease): CD
inflammation: Active
intestinal region: Sigmoid
Extracted molecule total RNA
Extraction protocol Pinch biopsies were obtained at colonoscopy using sterile cold biopsy forceps and snap-frozen for RNA extraction in Trizol reagent (Invitrogen). RNA was isolated using chloroform purification and isopropanol extraction and purified using QIAgen Rneasy Mini Kit.
Label Cy3
Label protocol Labeling was performed with Cy3 according to the manufacturer protoocl for one color microarray based gene expression analysis (Agilent)
 
Hybridization protocol Equal amount of Cy3 labeled target was hybridized to Sureprint G3 Human GE 8x60k array (Agilent) for 15 hours according to the manufacturers protocol
Scan protocol Arrays were scanned using Agilent microarray scanner
Data processing Raw signal intensities were extracted with feature extraction v11.5 software
 
Submission date Mar 15, 2017
Last update date Nov 12, 2017
Contact name Mei San Tang
Organization name Washington University in St Louis
Department Pathology & Immunology
Street address 660 S Euclid, Campus Box 8118
City Saint Louis
State/province MO
ZIP/Postal code 63108
Country USA
 
Platform ID GPL13607
Series (2)
GSE96665 Integrated analysis Of Biopsies From Inflammatory Bowel Disease Patients Identifies SAA1 As A Link Between Mucosal Microbes With TH17 And TH22 Cells [diseased subjects]
GSE97012 Integrated Analysis Of Biopsies From Inflammatory Bowel Disease Patients Identifies SAA1 As A Link Between Mucosal Microbes With TH17 And TH22 Cells

Data table header descriptions
ID_REF
VALUE Expression intensities were background corrected using the normal plus exponential convolutional method, followed by between-array quantile normalization in limma (version 3.26.9), resulting in log2-transformed intensity values.

Data table
ID_REF VALUE
1 16.98748218
2 5.069568709
3 5.280995466
4 10.6151779
5 9.014985667
6 9.94436742
7 13.11353919
8 13.56236274
9 11.12655259
10 7.739190856
11 5.163322716
12 12.98195459
13 11.51234287
14 12.27416059
15 12.20727599
16 5.95924445
17 7.568722698
18 8.89424517
19 8.694530246
20 10.59828794

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.




Supplementary file Size Download File type/resource
GSM2537698_US93503718_252800417818_S01_GE1_107_Sep09_2_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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