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Status |
Public on Sep 12, 2018 |
Title |
E9.5_Dgcr8-cKO+miR-541_rep2 |
Sample type |
SRA |
|
|
Source name |
E9.5_Dgcr8-cKO+miR-541
|
Organism |
Mus musculus |
Characteristics |
strain background: C57BL/6 genotype/variation: Mesp1Cre/+/Dgcr8-/- age: Embryonic day 9.5 transfected with: miR-541 mimics cell type: cultured heart cells
|
Treatment protocol |
In vitro cultured Dgcr8 cKO heart cells were transfected with miR-541-5p and NC miRNA 24 hrs after plating, and total RNA were collected 48 hrs after transfection
|
Growth protocol |
Dgcr8 cKO heart cells were dissociated into very small clusters, and cultured in vitro in a chemical defined medium
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TRIZOL and treated with RNase free DNase. RNA library was prepared following the instruction of SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing(Clontech)
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Description |
cultured heart cells
|
Data processing |
Sequenced reads were trimmed for adaptor sequences, and masked for low-complexity or low-quality sequences, then mapped to mm10 genome using STAR. Fragments Per Kilobase per Million mapped reads (FPKM) of Refseq genes were caculated. Genome_build: mm10 Supplementary_files_format_and_content: Excel files of gene names and FPKM values for each sample.
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|
|
Submission date |
Mar 15, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Jie Na |
E-mail(s) |
jie.na@tsinghua.edu.cn
|
Phone |
+86-18618192150
|
Organization name |
Tsinghua University
|
Department |
Basic Medical Sciences, School of Medicine
|
Lab |
D115 School of Medicine
|
Street address |
Tsinghua University
|
City |
Beijing |
State/province |
Beijing |
ZIP/Postal code |
100084 |
Country |
China |
|
|
Platform ID |
GPL21273 |
Series (2) |
GSE96647 |
Next Generation Sequencing Analysis of gene expression profile in E9.5 Mesp1Cre/+/Dgcr8-/- embryonic heart cells transfected with NC miRNA and miR-541 mimics |
GSE96648 |
Transcriptome analysis of mouse primitive heart with Dgcr8 deletion |
|
Relations |
BioSample |
SAMN06603958 |
SRA |
SRX2642196 |