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Sample GSM2537172 Query DataSets for GSM2537172
Status Public on Sep 12, 2018
Title E9.5_Dgcr8-cKO+NC-miRNA_rep1
Sample type SRA
 
Source name E9.5_Dgcr8-cKO+NC-miRNA
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: Mesp1Cre/+/Dgcr8-/-
age: Embryonic day 9.5
transfected with: NC miRNA
cell type: cultured heart cells
Treatment protocol In vitro cultured Dgcr8 cKO heart cells were transfected with miR-541-5p and NC miRNA 24 hrs after plating, and total RNA were collected 48 hrs after transfection
Growth protocol Dgcr8 cKO heart cells were dissociated into very small clusters, and cultured in vitro in a chemical defined medium
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIZOL and treated with RNase free DNase.
RNA library was prepared following the instruction of SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing(Clontech)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Description cultured heart cells
Data processing Sequenced reads were trimmed for adaptor sequences, and masked for low-complexity or low-quality sequences, then mapped to mm10 genome using STAR.
Fragments Per Kilobase per Million mapped reads (FPKM) of Refseq genes were caculated.
Genome_build: mm10
Supplementary_files_format_and_content: Excel files of gene names and FPKM values for each sample.
 
Submission date Mar 15, 2017
Last update date May 15, 2019
Contact name Jie Na
E-mail(s) jie.na@tsinghua.edu.cn
Phone +86-18618192150
Organization name Tsinghua University
Department Basic Medical Sciences, School of Medicine
Lab D115 School of Medicine
Street address Tsinghua University
City Beijing
State/province Beijing
ZIP/Postal code 100084
Country China
 
Platform ID GPL21273
Series (2)
GSE96647 Next Generation Sequencing Analysis of gene expression profile in E9.5 Mesp1Cre/+/Dgcr8-/- embryonic heart cells transfected with NC miRNA and miR-541 mimics
GSE96648 Transcriptome analysis of mouse primitive heart with Dgcr8 deletion
Relations
BioSample SAMN06603940
SRA SRX2642193

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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