|
| Status |
Public on Oct 11, 2017 |
| Title |
IL-36α stimuated keratinocytes rep3 |
| Sample type |
SRA |
| |
|
| Source name |
Keratinocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: keratinocytes
agent: IL-36α
passages: passage 2
|
| Treatment protocol |
Keratinocytes were treated with 20ng/ml IL-36α, IL-36β, IL-36γ (Bio-Techne) or vehicle control. Cells were harvested after 24h.
|
| Growth protocol |
Primary keratinocyte cultures were established as described elsewhere (Mahil et al, J Invest Dermatol 2016) using discarded healthy skin from a donor undergoing a plastic surgery procedure at Guy’s and St Thomas’ NHS Foundation Trust. Keratinocytes were cultured in Epilife medium containing Supplement 7 and 1% penicillin-streptomycin (all reagents Gibco).
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Plus Mini Kit (Qiagen)
RNAseq libraries were prepared with the Illumina TruSeq Stranded Total RNA preparation kit and sequenced on an Illumina HiSeq3000 system.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
A20_4
|
| Data processing |
Sequenced reads were aligned to the Ensembl Human GRCh37 transcriptome using Bowtie 2-2.2.2. The number of reads that mapped to each gene was quantified with htseq-count.
Genome_build: GRCh37
Supplementary_files_format_and_content: tab delimited file containing DESeq2 normalized counts
|
| |
|
| Submission date |
Mar 14, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Francesca Capon |
| E-mail(s) |
francesca.capon@kcl.ac.uk
|
| Organization name |
King's College London
|
| Department |
Genetics and Molecular Medicine
|
| Street address |
Great Maze Pond
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
| GSE96601 |
RNAseq of IL-36 stimulated primary human keratinocytes |
|
| Relations |
| BioSample |
SAMN06602278 |
| SRA |
SRX2639355 |
