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Sample GSM2486643 Query DataSets for GSM2486643
Status Public on Sep 01, 2017
Title HepG2_BFA_rep3
Sample type RNA
Source name HepG2, BFA
Organism Homo sapiens
Characteristics tissue: liver
cell line: HepG2
agent: BFA
Treatment protocol HepG2 cells were treated with DMSO or Brefeldin A (300 nM) for 24 hours.
Growth protocol HepG2 cells were grown in EMEM supplemented with 10% FBS (Thermo Fisher Scientific) and antibiotic/antimycotic solution (Sigma).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen).
Label Cy3
Label protocol 200 μg of total RNA were amplified and labeled using the Low Input QuickAmp Labeling kit (Agilent Technologies) according to the manufacturer’s protocols.
Hybridization protocol 600 ng of Cy3-labeled RNA was hybridized at 65⁰C for 17 hours.
Scan protocol The signals were detected by using the DNA microarray scanner (Agilent Technologies).
Description Gene expression in HepG2 cells treated with BFA
Data processing The scanned images were extracted with Feature Extraction software (Version; Agilent Technologies) using default parameters.
Submission date Feb 14, 2017
Last update date Sep 01, 2017
Contact name Kiyoshi Yamaguchi
Organization name The University of Tokyo
Department Division of Clinical Genome Research
Street address 4-6-1 Shirokanedai, Minato-ku
City Tokyo
ZIP/Postal code 108-8639
Country Japan
Platform ID GPL17077
Series (1)
GSE94861 Gene expression profiling of HepG2 cells after treatment with Brefeldin A

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_23_P117082 -0.6950426
A_33_P3246448 1.201334
A_33_P3318220 0.10238075
A_33_P3236322 0
A_33_P3319925 -0.099345446
A_21_P0000509 0.001151085
A_21_P0000744 1.2486544
A_24_P215804 0.118033886
A_23_P110167 -1.9177904
A_33_P3211513 1.03408
A_23_P103349 0.11153126
A_32_P61480 0.07604313
A_33_P3788124 0.13861513
A_33_P3414202 -1.8782806
A_33_P3316686 0.49430418
A_33_P3300975 0.14877462
A_33_P3263061 -0.43144083
A_33_P3261373 -0.18264532
A_24_P278460 0.083519936
A_21_P0013109 0.029957294

Total number of rows: 50737

Table truncated, full table size 1190 Kbytes.

Supplementary file Size Download File type/resource
GSM2486643_160914_BFA_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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