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Sample GSM2486641 Query DataSets for GSM2486641
Status Public on Sep 01, 2017
Title HepG2_BFA_rep1
Sample type RNA
 
Source name HepG2, BFA
Organism Homo sapiens
Characteristics tissue: liver
cell line: HepG2
agent: BFA
Treatment protocol HepG2 cells were treated with DMSO or Brefeldin A (300 nM) for 24 hours.
Growth protocol HepG2 cells were grown in EMEM supplemented with 10% FBS (Thermo Fisher Scientific) and antibiotic/antimycotic solution (Sigma).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen).
Label Cy3
Label protocol 200 μg of total RNA were amplified and labeled using the Low Input QuickAmp Labeling kit (Agilent Technologies) according to the manufacturer’s protocols.
 
Hybridization protocol 600 ng of Cy3-labeled RNA was hybridized at 65⁰C for 17 hours.
Scan protocol The signals were detected by using the DNA microarray scanner (Agilent Technologies).
Description Gene expression in HepG2 cells treated with BFA
Data processing The scanned images were extracted with Feature Extraction software (Version 10.7.3.1; Agilent Technologies) using default parameters.
 
Submission date Feb 14, 2017
Last update date Sep 01, 2017
Contact name Kiyoshi Yamaguchi
Organization name The University of Tokyo
Department Division of Clinical Genome Research
Street address 4-6-1 Shirokanedai, Minato-ku
City Tokyo
ZIP/Postal code 108-8639
Country Japan
 
Platform ID GPL17077
Series (1)
GSE94861 Gene expression profiling of HepG2 cells after treatment with Brefeldin A

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_23_P117082 -0.6793318
A_33_P3246448 0.01313448
A_33_P3318220 0.011276245
A_33_P3236322 -0.096390724
A_33_P3319925 0.06057787
A_21_P0000509 -0.17717457
A_21_P0000744 1.2418814
A_24_P215804 0
A_23_P110167 -2.037403
A_33_P3211513 1.0142221
A_23_P103349 -0.001726627
A_32_P61480 0.2360487
A_33_P3788124 0
A_33_P3414202 -1.7608194
A_33_P3316686 0.31841898
A_33_P3300975 0
A_33_P3263061 -0.47243452
A_33_P3261373 0
A_24_P278460 -0.07388401
A_21_P0013109 -0.49222565

Total number of rows: 50737

Table truncated, full table size 1176 Kbytes.




Supplementary file Size Download File type/resource
GSM2486641_160914_BFA_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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