|
| Status |
Public on Aug 22, 2017 |
| Title |
YAPTAZ-ECKO2 |
| Sample type |
SRA |
| |
|
| Source name |
Brain endothelial cells (Brain ECs)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: FACS sorted Brain ECs
age: P8
genotype: VE-cadherin-CreERT2 Yapf/f Tazf/f
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using fastx_trimmer), then mapped to mm10 whole genome using Bowtie2
Read count extraction and nomalization were performed using edgeR.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include read count values for each Sample
|
| |
|
| Submission date |
Feb 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Hosung Bae |
| Organization name |
UC Irvine
|
| Street address |
839 Health Sciences Road Sprague Hall, Room 150
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE94673 |
RNA-sequencing of brain ECs isolated at P8 from mice with EC-specific deletion of YAP/TAZ |
|
| Relations |
| BioSample |
SAMN06312274 |
| SRA |
SRX2545080 |
