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Sample GSM247505 Query DataSets for GSM247505
Status Public on Dec 07, 2007
Title MBG treated 12 hr (M_1.2 12)
Sample type RNA
 
Source name Human Dermal Fibroblast
Organism Homo sapiens
Characteristics Cell line
Treatment protocol Cell were treated with 10 nM of marinobufagenin (MBG) for the indicated times follows by RNA isolation.
Growth protocol Human skin fibroblasts, obtained from Cambrex, were cultured at 37oC in 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Qiagen's Rneasy column according to manufacturer's specification.
Label Cy5
Label protocol cDNA were synthesized from the isolated total RNA by reverse transcription followed by second strand synthesis using the Amino Allyl MessageAmp II aRNA Amplification kit (Ambion, Austin, TX) with oligo(dT) primer that contain a T7 RNA polymerase promoter sequence, according to the manufacturer’s specification. In vitro transcription was then performed with the purified cDNA and amino allyl UTP was incorporated during transcription to produce amino allyl modified aRNA that was subsequently coupled to cy5 dye label.
 
Hybridization protocol The HumanOneArray was hybridized using 2X Gex Hybridization Buffer (Agilent) for 17 h at 65oC; then washed in Gene Expression Wash Buffer 1 and 2 (Agilent) per manufacturer's instruction.
Scan protocol The arrays were scanned with GenePix 4000B array scanner, images of the hybridized arrays were processed BioDiscovery's ImaGene and signal intensities quantified.
Description Duplicate
Data processing The raw data were re-scaled to account for the differences in individual hybridization intensities. We employed a rank-invariant scaling method to select genes (Tseng et al., 2001), which were then used for fitting of a non-linear normalization curve.
 
Submission date Dec 06, 2007
Last update date Aug 14, 2011
Contact name Khew-Voon Chin
E-mail(s) khew-voon.chin@utoledo.edu
Phone 419-383-3502
Fax 419-383-4473
Organization name The University of Toledo College of Medicine
Department Medicine
Street address 3000 Arlington Avenue
City Toledo
State/province OH
ZIP/Postal code 43614
Country USA
 
Platform ID GPL6254
Series (1)
GSE9806 A Time Course Analysis of the Effects of Marinobufagenin in Human Dermal Fibroblasts

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
PH_hs_0000002 8
PH_hs_0000003 18
PH_hs_0000004 1414
PH_hs_0000005 98
PH_hs_0000006 36
PH_hs_0000007 647
PH_hs_0000008 499
PH_hs_0000009 803
PH_hs_0000010 2363
PH_hs_0000011 41
PH_hs_0000012 39
PH_hs_0000013 113
PH_hs_0000014 1077
PH_hs_0000015 20
PH_hs_0000016 13
PH_hs_0000017 21
PH_hs_0000018 702
PH_hs_0000019 34
PH_hs_0000020 51375
PH_hs_0000021 44

Total number of rows: 30968

Table truncated, full table size 524 Kbytes.




Supplementary file Size Download File type/resource
GSM247505.gpr.gz 2.7 Mb (ftp)(http) GPR
Processed data included within Sample table

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