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Sample GSM247501 Query DataSets for GSM247501
Status Public on Dec 07, 2007
Title MBG treated 2 hr (M_1.2 2)
Sample type RNA
 
Source name Human Dermal Fibroblast
Organism Homo sapiens
Characteristics Cell line
Treatment protocol Cell were treated with 10 nM of marinobufagenin (MBG) for the indicated times follows by RNA isolation.
Growth protocol Human skin fibroblasts, obtained from Cambrex, were cultured at 37oC in 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Qiagen's Rneasy column according to manufacturer's specification.
Label Cy5
Label protocol cDNA were synthesized from the isolated total RNA by reverse transcription followed by second strand synthesis using the Amino Allyl MessageAmp II aRNA Amplification kit (Ambion, Austin, TX) with oligo(dT) primer that contain a T7 RNA polymerase promoter sequence, according to the manufacturer’s specification. In vitro transcription was then performed with the purified cDNA and amino allyl UTP was incorporated during transcription to produce amino allyl modified aRNA that was subsequently coupled to cy5 dye label.
 
Hybridization protocol The HumanOneArray was hybridized using 2X Gex Hybridization Buffer (Agilent) for 17 h at 65oC; then washed in Gene Expression Wash Buffer 1 and 2 (Agilent) per manufacturer's instruction.
Scan protocol The arrays were scanned with GenePix 4000B array scanner, images of the hybridized arrays were processed BioDiscovery's ImaGene and signal intensities quantified.
Description Duplicate
Data processing The raw data were re-scaled to account for the differences in individual hybridization intensities. We employed a rank-invariant scaling method to select genes (Tseng et al., 2001), which were then used for fitting of a non-linear normalization curve.
 
Submission date Dec 06, 2007
Last update date Aug 14, 2011
Contact name Khew-Voon Chin
E-mail(s) khew-voon.chin@utoledo.edu
Phone 419-383-3502
Fax 419-383-4473
Organization name The University of Toledo College of Medicine
Department Medicine
Street address 3000 Arlington Avenue
City Toledo
State/province OH
ZIP/Postal code 43614
Country USA
 
Platform ID GPL6254
Series (1)
GSE9806 A Time Course Analysis of the Effects of Marinobufagenin in Human Dermal Fibroblasts

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
PH_hs_0000002 48
PH_hs_0000003 29
PH_hs_0000004 1052
PH_hs_0000005 81
PH_hs_0000006 67
PH_hs_0000007 527
PH_hs_0000008 545
PH_hs_0000009 374
PH_hs_0000010 2306
PH_hs_0000011 66
PH_hs_0000012 67
PH_hs_0000013 144
PH_hs_0000014 819
PH_hs_0000015 83
PH_hs_0000016 1
PH_hs_0000017 20
PH_hs_0000018 623
PH_hs_0000019 36
PH_hs_0000020 64581
PH_hs_0000021 61

Total number of rows: 30968

Table truncated, full table size 529 Kbytes.




Supplementary file Size Download File type/resource
GSM247501.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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