|
Status |
Public on Sep 24, 2017 |
Title |
H3K27Ac_d120 |
Sample type |
SRA |
|
|
Source name |
MCF10A
|
Organism |
Homo sapiens |
Characteristics |
chip antibody: Histone 3 K27 Acetylation - (ab4729, abcam) treatment: dexamethasone (DEX) treatemtn time: 120 min
|
Treatment protocol |
Cells were starved overnight in medium without additives and then stimulated with EGF (e), DEX (d) or the combination (ed) for the indicated time intervals (minutes) before sequencing
|
Growth protocol |
MCF10A cells were cultured as described (Tarcic et al., 2012) in DME:F12 medium (Gibco BRL, Grand Island, NY) supplemented with 10 µg ml-1 insulin, 0.1 µg ml-1 cholera toxin, 0.5 µg ml-1 hydrocortison, 5% heat-inactivated horse serum (Biological Industries, Beit-Haemek, Israel) and 10 ng ml-1 EGF.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Cell fixation and chromatin immunoprecipitation were performed as described (Blecher-Gonen et al., 2013) Library construction and sequencing were performed as described (Blecher-Gonen et al., 2013)
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Chromatin immunoprecipitation (ChIP) sequencing reads were mapped to the hg19 genome assembly using Bowtie2 (Langmead and Salzberg, 2012) Genome_build: hg19 Supplementary_files_format_and_content: Tag read densities were generated by the makeTagDirectory from the Homer suite
|
|
|
Submission date |
Feb 02, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Yehoshua Enuka |
E-mail(s) |
ys.enuka@gmail.com
|
Phone |
+972-54-2345623
|
Organization name |
Weizmann Institute of Science
|
Department |
Biological Regulation
|
Lab |
Yarden
|
Street address |
Herzl 234
|
City |
Rehovot |
ZIP/Postal code |
76100 |
Country |
Israel |
|
|
Platform ID |
GPL16791 |
Series (2) |
GSE94063 |
Epigenetic mechanisms underlie the crosstalk between growth factors and a steroid hormone |
GSE94406 |
Epigenetic mechanisms underlie the crosstalk between growth factors and a steroid hormone [H3K27Ac ChIP-Seq] |
|
Relations |
BioSample |
SAMN06290021 |
SRA |
SRX2533062 |