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Sample GSM2473476 Query DataSets for GSM2473476
Status Public on Jan 01, 2018
Title Patient 203 -skin - Placebo - Day28
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics timepoint: Day28
treatment: Placebo
Treatment protocol Each subject forearm was randomized to either treatment or placebo and treated for 28 days.
Extracted molecule total RNA
Extraction protocol Skin bioeise placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RLT Buffe and purifying over Rneasy columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing processed using affy package for R, using MAS5 normalization
 
Submission date Jan 31, 2017
Last update date Jan 01, 2018
Contact name Julio C Mantero
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE94340 Expression data from skin biopsies in patients with systemic sclerosis treated with beta-catenin inhibitor (C82) and placebo

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 9.925947715
1053_at 8.587288595
117_at 8.32222414
121_at 8.12804873
1255_g_at 3.381442386
1294_at 8.325894656
1316_at 6.938513655
1320_at 7.025058369
1405_i_at 7.841648075
1431_at 6.945320128
1438_at 7.547988662
1487_at 8.696376072
1494_f_at 5.766488282
1598_g_at 11.39500307
160020_at 9.916765532
1729_at 8.816207125
177_at 7.303921789
1773_at 7.305245208
179_at 7.446841794
1861_at 8.150133239

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM2473476_RL_E7_68.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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