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Sample GSM2473475 Query DataSets for GSM2473475
Status Public on Jan 01, 2018
Title Patient 203 -skin - C82 - Day28
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics timepoint: Day28
treatment: C82
Treatment protocol Each subject forearm was randomized to either treatment or placebo and treated for 28 days.
Extracted molecule total RNA
Extraction protocol Skin bioeise placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RLT Buffe and purifying over Rneasy columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing processed using affy package for R, using MAS5 normalization
 
Submission date Jan 31, 2017
Last update date Jan 01, 2018
Contact name Julio C Mantero
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE94340 Expression data from skin biopsies in patients with systemic sclerosis treated with beta-catenin inhibitor (C82) and placebo

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 9.947007865
1053_at 8.42400935
117_at 8.547372653
121_at 8.386105634
1255_g_at 4.129238161
1294_at 8.022329022
1316_at 6.856162017
1320_at 6.920004779
1405_i_at 7.955450579
1431_at 7.241529537
1438_at 7.573846757
1487_at 9.081291739
1494_f_at 5.206962837
1598_g_at 11.46695596
160020_at 9.620171562
1729_at 8.789560351
177_at 7.181139193
1773_at 7.259139374
179_at 7.410240881
1861_at 7.950348386

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM2473475_RL_E6_67.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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