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Sample GSM2473466 Query DataSets for GSM2473466
Status Public on Jan 01, 2018
Title Patient 201 -skin - C82 - Baseline
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics timepoint: Baseline
treatment: C82
Treatment protocol Each subject forearm was randomized to either treatment or placebo and treated for 28 days.
Extracted molecule total RNA
Extraction protocol Skin bioeise placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RLT Buffe and purifying over Rneasy columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing processed using affy package for R, using MAS5 normalization
 
Submission date Jan 31, 2017
Last update date Jan 01, 2018
Contact name Julio C Mantero
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE94340 Expression data from skin biopsies in patients with systemic sclerosis treated with beta-catenin inhibitor (C82) and placebo

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 9.566340596
1053_at 8.47649869
117_at 7.586082717
121_at 7.063414462
1255_g_at 4.148912112
1294_at 7.342648072
1316_at 6.767398743
1320_at 6.693718011
1405_i_at 8.634276268
1431_at 5.734095646
1438_at 7.574288782
1487_at 8.531011147
1494_f_at 4.568986193
1598_g_at 11.43848043
160020_at 10.25596839
1729_at 8.258442212
177_at 7.856615653
1773_at 7.202583337
179_at 7.013673059
1861_at 7.831957213

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM2473466_RL_D5_58.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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