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Sample GSM2473456 Query DataSets for GSM2473456
Status Public on Jan 01, 2018
Title Patient 305 -skin - C82 - Day28
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics timepoint: Day28
treatment: C82
Treatment protocol Each subject forearm was randomized to either treatment or placebo and treated for 28 days.
Extracted molecule total RNA
Extraction protocol Skin bioeise placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RLT Buffe and purifying over Rneasy columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing processed using affy package for R, using MAS5 normalization
 
Submission date Jan 31, 2017
Last update date Jan 01, 2018
Contact name Julio C Mantero
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE94340 Expression data from skin biopsies in patients with systemic sclerosis treated with beta-catenin inhibitor (C82) and placebo

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 9.76043425
1053_at 8.630332903
117_at 7.536842538
121_at 7.762179526
1255_g_at 3.863768748
1294_at 7.305511633
1316_at 7.140345166
1320_at 7.084715734
1405_i_at 8.104602953
1431_at 7.831590403
1438_at 7.241749123
1487_at 8.466450804
1494_f_at 4.619450913
1598_g_at 11.43304142
160020_at 9.554361339
1729_at 8.358365424
177_at 7.547138888
1773_at 6.984632188
179_at 6.499828044
1861_at 8.328710057

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM2473456_RL_C7_52w.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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