NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2473436 Query DataSets for GSM2473436
Status Public on Jan 01, 2018
Title Patient 108 -skin - Placebo - Baseline
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics timepoint: Baseline
treatment: Placebo
Treatment protocol Each subject forearm was randomized to either treatment or placebo and treated for 28 days.
Extracted molecule total RNA
Extraction protocol Skin bioeise placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RLT Buffe and purifying over Rneasy columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing processed using affy package for R, using MAS5 normalization
 
Submission date Jan 31, 2017
Last update date Jan 01, 2018
Contact name Julio C Mantero
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE94340 Expression data from skin biopsies in patients with systemic sclerosis treated with beta-catenin inhibitor (C82) and placebo

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 10.28143633
1053_at 8.390554293
117_at 6.894065527
121_at 8.256284516
1255_g_at 4.417572708
1294_at 7.145120918
1316_at 6.946622664
1320_at 7.063358375
1405_i_at 6.05485119
1431_at 6.765363314
1438_at 7.706742021
1487_at 8.562909303
1494_f_at 5.427331698
1598_g_at 10.69707023
160020_at 9.625808216
1729_at 8.703096126
177_at 6.994733986
1773_at 7.566111021
179_at 6.379613348
1861_at 8.374995483

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM2473436_RL_B5_30.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap