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Sample GSM2473435 Query DataSets for GSM2473435
Status Public on Jan 01, 2018
Title Patient 104 -skin - Placebo - Baseline
Sample type RNA
 
Source name human skin
Organism Homo sapiens
Characteristics timepoint: Baseline
treatment: Placebo
Treatment protocol Each subject forearm was randomized to either treatment or placebo and treated for 28 days.
Extracted molecule total RNA
Extraction protocol Skin bioeise placed in RNAlater at bedside were stored at -80 degrees C until processing. RNA was exrtacted by homogenization in RLT Buffe and purifying over Rneasy columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The labeled, fragmented DNA was hybridized to the GeneChip HG-U133A 2.0 Array (Affymetrix, Santa Clara, CA) for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450
Scan protocol After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix, Santa Clara, CA).
Data processing processed using affy package for R, using MAS5 normalization
 
Submission date Jan 31, 2017
Last update date Jan 01, 2018
Contact name Julio C Mantero
Organization name Boston University School of Medicine
Department Rheumatology
Street address 72 E Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platform ID GPL571
Series (1)
GSE94340 Expression data from skin biopsies in patients with systemic sclerosis treated with beta-catenin inhibitor (C82) and placebo

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 9.719978072
1053_at 8.643198407
117_at 7.497217528
121_at 7.804820437
1255_g_at 4.667045662
1294_at 6.988068665
1316_at 6.067645584
1320_at 7.449362164
1405_i_at 8.23076303
1431_at 6.983998156
1438_at 7.301148588
1487_at 8.763787727
1494_f_at 5.38559125
1598_g_at 11.39315054
160020_at 9.157578948
1729_at 8.923890858
177_at 7.425724727
1773_at 7.328150251
179_at 7.253439888
1861_at 8.175570815

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM2473435_RL_B5_13.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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