|
Status |
Public on Aug 08, 2017 |
Title |
10h LPS rep2 |
Sample type |
SRA |
|
|
Source name |
10h LPS
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J cell type: Bone marrow derived macrophages treatment: 10h LPS
|
Treatment protocol |
Primary macrophages derived from bone marrow were stimulated with lipopolysaccharide and/or Dex for the indicated number of hours.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from approximately 1x10e6 cells by using an RNAeasy Mini Kit (Qiagen). Sequencing libraries were generated using Illumina TruSeq V3 protocol and subject to 101 bp paired-end sequencing on Illumina HiSeq2000.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
GH1291
|
Data processing |
Base-calling with Illumina run time analysis software 1.12.4.2 Alignment to mm9 using Casava Eland extended Transcript levels were quantified using Tophat and Cufflinks. Genome_build: mm9 Supplementary_files_format_and_content: FPKM
|
|
|
Submission date |
Jan 17, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Songjoon Baek |
Organization name |
NCI / NIH
|
Department |
CCR
|
Lab |
LRBGE
|
Street address |
41 Library Drive
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL13112 |
Series (2) |
GSE93735 |
Anti-inflammatory Chromatinscape Associated with Clinically Relevant Timing of Glucocorticoid Treatment [RNA-Seq] |
GSE93739 |
Anti-inflammatory Chromatinscape Associated with Clinically Relevant Timing of Glucocorticoid Treatment |
|
Relations |
BioSample |
SAMN06236688 |
SRA |
SRX2498769 |