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Sample GSM2461315 Query DataSets for GSM2461315
Status Public on Aug 08, 2017
Title 10h LPS rep2
Sample type SRA
 
Source name 10h LPS
Organism Mus musculus
Characteristics strain: C57BL/6J
cell type: Bone marrow derived macrophages
treatment: 10h LPS
Treatment protocol Primary macrophages derived from bone marrow were stimulated with lipopolysaccharide and/or Dex for the indicated number of hours.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from approximately 1x10e6 cells by using an RNAeasy Mini Kit (Qiagen).
Sequencing libraries were generated using Illumina TruSeq V3 protocol and subject to 101 bp paired-end sequencing on Illumina HiSeq2000.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description GH1291
Data processing Base-calling with Illumina run time analysis software 1.12.4.2
Alignment to mm9 using Casava Eland extended
Transcript levels were quantified using Tophat and Cufflinks.
Genome_build: mm9
Supplementary_files_format_and_content: FPKM
 
Submission date Jan 17, 2017
Last update date May 15, 2019
Contact name Songjoon Baek
Organization name NCI / NIH
Department CCR
Lab LRBGE
Street address 41 Library Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL13112
Series (2)
GSE93735 Anti-inflammatory Chromatinscape Associated with Clinically Relevant Timing of Glucocorticoid Treatment [RNA-Seq]
GSE93739 Anti-inflammatory Chromatinscape Associated with Clinically Relevant Timing of Glucocorticoid Treatment
Relations
BioSample SAMN06236688
SRA SRX2498769

Supplementary file Size Download File type/resource
GSM2461315_GH1291.genes.fpkm_tracking.gz 753.2 Kb (ftp)(http) FPKM_TRACKING
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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