The PAXgene Blood RNA Kit (PreAnalytiX) was used to isolate RNA according to the manufacturer’s protocol.
Label
biotin
Label protocol
Samples were fragmented and labelled using the Encore® Biotin Module Kit (NuGEN)
Hybridization protocol
Samples were hybridized using Affymetrix hybridization kit material.
Scan protocol
Affymetrix GeneTitan
Description
IPF, Timepoint 0
Data processing
Data were processed using Affymetrix Power Tools. RMA background correction were applied to probe intensities, gene level expression were summarized from the intensities and then quantile normalized.
