NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM243745 Query DataSets for GSM243745
Status Public on Dec 18, 2008
Title HMEC Ctrl 4
Sample type RNA
 
Source name Human Mammary Epithelial Cell
Organism Homo sapiens
Characteristics HMEC in control media under normoxia
Treatment protocol The first set of experiments were done in triplicate by exposing cells to control, 25mM lactic acidosis of pH6.7, 2%hypoxia, and the combination of lactic acidosis and hypoxia. The second set of experiments were done in replica of six replicates and cells were exposed to control, acidosis with the addition of HCl to reach pH 6.7 and 25mM sodium lactate.
Growth protocol Human mammary epithelial cells were induced into replicative arrest by growth factor/serum withdrawl for 24 hrs and then exposed to different cultural environments.
Extracted molecule total RNA
Extraction protocol RNAs were extracted with miRVana kit from Ambion
Label biotin
Label protocol Affymetrix standard protocol
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description gene expression data from HMEC cells
Data processing Data was normalized with RMA and MAS5 and further analyzed with hierarchical clustering as well as binary regression.
 
Submission date Nov 20, 2007
Last update date Aug 28, 2018
Contact name Julia, Ling-Yu Chen
Organization name Duke university
Street address CIEMAS Rm.2141, 101 Science Dr.
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL570
Series (1)
GSE9649 Expression studies of HMEC exposed to lactic acidosis and hypoxia
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 12.32520564
1053_at 7.172184555
117_at 6.311845621
121_at 9.483287867
1255_g_at 3.872042572
1294_at 7.683826347
1316_at 6.029719063
1320_at 6.43649419
1405_i_at 3.895264529
1431_at 4.3940655
1438_at 9.367644054
1487_at 8.316035899
1494_f_at 7.260877804
1552256_a_at 9.907036783
1552257_a_at 9.120692922
1552258_at 5.591966866
1552261_at 6.5117893
1552263_at 6.919347969
1552264_a_at 8.828463278
1552266_at 3.810433541

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM243745.CEL.gz 8.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap