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Sample GSM243739 Query DataSets for GSM243739
Status Public on Dec 18, 2008
Title HMEC Lactic Acidosis plus hypoxia 1
Sample type RNA
 
Source name Human Mammary Epithelial Cell
Organism Homo sapiens
Characteristics HMEC in media with 25mM lactic acidosis under hypoxia
Treatment protocol The first set of experiments were done in triplicate by exposing cells to control, 25mM lactic acidosis of pH6.7, 2%hypoxia, and the combination of lactic acidosis and hypoxia. The second set of experiments were done in replica of six replicates and cells were exposed to control, acidosis with the addition of HCl to reach pH 6.7 and 25mM sodium lactate.
Growth protocol Human mammary epithelial cells were induced into replicative arrest by growth factor/serum withdrawl for 24 hrs and then exposed to different cultural environments.
Extracted molecule total RNA
Extraction protocol RNAs were extracted with miRVana kit from Ambion
Label biotin
Label protocol Affymetrix standard protocol
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description gene expression data from HMEC cells
Data processing Data was normalized with RMA and MAS5 and further analyzed with hierarchical clustering as well as binary regression.
 
Submission date Nov 20, 2007
Last update date Aug 28, 2018
Contact name Julia, Ling-Yu Chen
Organization name Duke university
Street address CIEMAS Rm.2141, 101 Science Dr.
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL570
Series (1)
GSE9649 Expression studies of HMEC exposed to lactic acidosis and hypoxia
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 13.09099326
1053_at 8.132836666
117_at 7.368024319
121_at 10.17665838
1255_g_at 4.732055091
1294_at 7.890058094
1316_at 7.025825013
1320_at 6.896878973
1405_i_at 5.738654313
1431_at 5.327306585
1438_at 11.35832691
1487_at 9.044813633
1494_f_at 8.403646375
1552256_a_at 10.37352598
1552257_a_at 9.889665225
1552258_at 6.822836749
1552261_at 6.911104747
1552263_at 7.278202261
1552264_a_at 9.962611728
1552266_at 5.934233934

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM243739.CEL.gz 8.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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