|
| Status |
Public on Nov 24, 2007 |
| Title |
head and neck squamous cell carcinoma (HNSCC) sampleNo.10 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HNSCC10N
|
| Organism |
Homo sapiens |
| Characteristics |
Non-tumorus tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol procedure
|
| Label |
Cy3
|
| Label protocol |
Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp™ aRNA Amplification Kit (Ambion). CyeDye Coupling and fragmentation were performed as the protocol supplied by Hitach Software Engineering Co., Ltd. http://www.dna-chip.co.jp/thesis/AceGeneProtocol.pdf
|
| |
|
| Channel 2 |
| Source name |
HNSCC10T
|
| Organism |
Homo sapiens |
| Characteristics |
Tumor tissue
Age:58
Gender:Male
Histlogical Type:well differentiated squamous cell carcinoma
Stage:ⅣA (T3N2c)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol procedure
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp™ aRNA Amplification Kit (Ambion). CyeDye Coupling and fragmentation were performed as the protocol supplied by Hitach Software Engineering Co., Ltd. http://www.dna-chip.co.jp/thesis/AceGeneProtocol.pdf
|
| |
|
| |
| Hybridization protocol |
Hybridized for 20 h at 50 C. Hybridization buffer and washing protocol was followed by the protocol supplied by Hitach Software Engineering Co., Ltd. http://www.dna-chip.co.jp/thesis/AceGeneProtocol.pdf
|
| Scan protocol |
ScanArray HT (PerkinElmer Japan Co., Ltd.) was used for scanning. Array images were analyzed with DNASIS Array (Hitachi Software Engineering Co., Ltd.).
|
| Description |
HNSCC10
|
| Data processing |
This data were analyzed by DNASIS array software(Hitachi Software Engineering), which converted the signal intensity of each spot into text format. Log2-ratios of the median subtracted background intensity levels were analyzed. Data from each microarray were normalized by LOWESS normalization.
|
| |
|
| Submission date |
Nov 16, 2007 |
| Last update date |
May 15, 2019 |
| Contact name |
Satoya Shimizu |
| E-mail(s) |
smzsty@graduate.chiba-u.jp
|
| Organization name |
Chiba university
|
| Street address |
1-8-1 Inohana, Chuo-ku
|
| City |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1291 |
| Series (1) |
| GSE9638 |
Gene expression profiles of head and neck squamous cell carcinoma (HNSCC) |
|
| Relations |
| SRA |
SRX2386240 |
