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Sample GSM2433812 Query DataSets for GSM2433812
Status Public on May 29, 2018
Title E6.5A
Sample type SRA
 
Source name Anterior embryonic tissue dissected from E6.5 embryo
Organism Mus musculus
Characteristics strain: ICR
tissue: Anterior embryonic tissue
Stage: Embryonic day 6.5
Extracted molecule total RNA
Extraction protocol For cell lines, total RNAs were extracted by using Trizol (Invitrogen) according to the manufacturer’s instructions. For embryonic sample, small tissue sample were dissolved in lysis solution, and reverse transcription was then performed. After the 1st-strand synthesis, cDNA was directly preamplified with KAPA HiFi Hotstart ReadyMix (KAPA Biosystems) by IS-PCR primer for 18 cycles
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description embryo.gene.fpkm.txt
Data processing Raw reads were mapped to mm10 using the TopHat version 2.0.13 program
We assigned FPKM (fragment per kilo base per million) as an expression value for each gene using Cufflinks version 1.3.0 software
Cuffdiff software was used to identify differentially expressed genes between treatment and control samples
Genome_build: mm10
Supplementary_files_format_and_content: FPKM values for each gene in each Sample
 
Submission date Dec 20, 2016
Last update date May 15, 2019
Contact name Naihe Jing
E-mail(s) njing@sibcb.ac.cn
Organization name Shanghai Institute of Biochemistry and Cell Biology
Street address Yue Yang Road 320
City Shanghai
ZIP/Postal code 200031
Country China
 
Platform ID GPL13112
Series (2)
GSE92634 RNA sequencing of Epiblast stem cells, nodal inhibited epiblast stem cells and embryonic samples
GSE92635 Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells
Relations
BioSample SAMN06167409
SRA SRX2437198

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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