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Sample GSM2432629 Query DataSets for GSM2432629
Status Public on Mar 01, 2017
Title HCT116 DGKG-CA
Sample type RNA
Source name HCT116 cells overexpressing DGKG-CA
Organism Homo sapiens
Characteristics vector: DGKG-CA
cell line: HCT116
Treatment protocol HCT116 cells were infected with an adenoviral vector for expressing LacZ, or DGKG-KD or DGKG-CA. Twenty-four hours after infection, total RNA was extracted.
Growth protocol HCT116 cells were cultured in McCoy’s 5A medium supplemented with 10% heat-inactivated fetal bovine serum. The cultures were incubated at 37ºC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a TRIZOL reagent (Thermo Fisher Scientific).
Label Cy3
Label protocol Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
Hybridization protocol Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
Scan protocol Array was scanned with an Agilent G2565BA Microarray Scanner.
Description Gene expression in HCT116 cells overexpressing DGKG-CA
Data processing Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
Submission date Dec 19, 2016
Last update date Mar 02, 2017
Contact name Hiromu Suzuki
Phone +81-11-611-2111
Organization name Sapporo Medical University
Department Department of Molecular Biology
Street address S1, W17, Chuo-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8556
Country Japan
Platform ID GPL13607
Series (1)
GSE92573 Analysis of gene expression in colorectal cancer cells with DGKG overexpression

Data table header descriptions
VALUE Background subtracted signal

Data table
1 3.09E+04
2 5.29E+00
3 5.34E+00
4 1.36E+02
5 3.90E+02
6 7.03E+01
7 3.03E+03
8 6.14E+02
9 5.58E+00
10 3.44E+01
11 5.64E+00
12 8.23E+02
13 4.38E+02
14 3.30E+02
15 1.22E+04
16 5.74E+00
17 1.14E+02
18 8.25E+01
19 5.78E+00
20 5.39E+02

Total number of rows: 62976

Table truncated, full table size 911 Kbytes.

Supplementary file Size Download File type/resource
GSM2432629_HCT116_DGKG-CA_24h.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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