|
| Status |
Public on Mar 01, 2017 |
| Title |
HCT116 LacZ |
| Sample type |
RNA |
| |
|
| Source name |
HCT116 cells overexpressing LacZ
|
| Organism |
Homo sapiens |
| Characteristics |
vector: LacZ
cell line: HCT116
|
| Treatment protocol |
HCT116 cells were infected with an adenoviral vector for expressing LacZ, or DGKG-KD or DGKG-CA. Twenty-four hours after infection, total RNA was extracted.
|
| Growth protocol |
HCT116 cells were cultured in McCoy’s 5A medium supplemented with 10% heat-inactivated fetal bovine serum. The cultures were incubated at 37ºC in a humidified incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a TRIZOL reagent (Thermo Fisher Scientific).
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Description |
Gene expression in HCT116 cells overexpressing LacZ
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
|
| |
|
| Submission date |
Dec 19, 2016 |
| Last update date |
Mar 02, 2017 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
|
| Phone |
+81-11-611-2111
|
| Organization name |
Sapporo Medical University
|
| Department |
Department of Molecular Biology
|
| Street address |
S1, W17, Chuo-ku
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE92573 |
Analysis of gene expression in colorectal cancer cells with DGKG overexpression |
|
