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Sample GSM2432627 Query DataSets for GSM2432627
Status Public on Mar 01, 2017
Title HCT116 LacZ
Sample type RNA
Source name HCT116 cells overexpressing LacZ
Organism Homo sapiens
Characteristics vector: LacZ
cell line: HCT116
Treatment protocol HCT116 cells were infected with an adenoviral vector for expressing LacZ, or DGKG-KD or DGKG-CA. Twenty-four hours after infection, total RNA was extracted.
Growth protocol HCT116 cells were cultured in McCoy’s 5A medium supplemented with 10% heat-inactivated fetal bovine serum. The cultures were incubated at 37ºC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a TRIZOL reagent (Thermo Fisher Scientific).
Label Cy3
Label protocol Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
Hybridization protocol Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
Scan protocol Array was scanned with an Agilent G2565BA Microarray Scanner.
Description Gene expression in HCT116 cells overexpressing LacZ
Data processing Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
Submission date Dec 19, 2016
Last update date Mar 02, 2017
Contact name Hiromu Suzuki
Phone +81-11-611-2111
Organization name Sapporo Medical University
Department Department of Molecular Biology
Street address S1, W17, Chuo-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8556
Country Japan
Platform ID GPL13607
Series (1)
GSE92573 Analysis of gene expression in colorectal cancer cells with DGKG overexpression

Data table header descriptions
VALUE Background subtracted signal

Data table
1 3.20E+04
2 5.63E+00
3 5.65E+00
4 1.34E+02
5 8.20E+02
6 7.49E+01
7 3.11E+03
8 6.46E+02
9 5.76E+00
10 2.78E+01
11 5.78E+00
12 9.44E+02
13 4.76E+02
14 3.80E+02
15 1.57E+04
16 5.80E+00
17 1.74E+02
18 1.07E+02
19 5.79E+00
20 6.51E+02

Total number of rows: 62976

Table truncated, full table size 911 Kbytes.

Supplementary file Size Download File type/resource
GSM2432627_HCT116_LacZ_24h.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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