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Sample GSM2431176 Query DataSets for GSM2431176
Status Public on Dec 17, 2016
Title B6-1
Sample type genomic
 
Channel 1
Source name Foxp3-ChIP DNA from mouse Treg
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: CD4+CD25+ Treg
gender: male
age: 8 weeks
chip antibody: anti-Foxp3 antibody (eBioscience cat# 14-4774-82)
Growth protocol CD4+CD25+ Treg isolation
Extracted molecule genomic DNA
Extraction protocol genomic DNA isolation
Label Cy5 (635)
Label protocol Nimblegen manufacturer protocol
 
Channel 2
Source name Input DNA from CD4+CD25+ Treg
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: CD4+CD25+ Treg
gender: male
age: 8 weeks
Growth protocol CD4+CD25+ Treg isolation
Extracted molecule genomic DNA
Extraction protocol genomic DNA isolation
Label Cy3 (532)
Label protocol Nimblegen manufacturer protocol
 
 
Hybridization protocol Nimblegen manufacturer protocol
Scan protocol chip submitted to Nimblegen for scanning
Data processing Data were analyzed using Partek GS (Partek Inc., St Louis, MO) and the Broad Institutes Interactive Genome Viewer (Version 2.3.32) (Robinson et al., 2011). Raw data underwent Loess normalization, and Student t-testing with a false discovery rate of 0.1 (p-value cutoff 0.0029).
 
Submission date Dec 16, 2016
Last update date Dec 17, 2016
Contact name Wayne W Hancock
Organization name CHOP/UPenn
Street address 916B ARC, 3615 Civic Ctr Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104-4318
Country USA
 
Platform ID GPL21030
Series (1)
GSE92520 Foxp3 DNA binding in HDAC6-KO vs wild type Regulatory T cells

Data table header descriptions
ID_REF
VALUE log2 transformed ratio representing ChIP/input

Data table
ID_REF VALUE
CHR10FS100051103 0.579884
CHR10FS100051208 0.404296
CHR10FS100051318 0.375653
CHR10FS100051443 0.37918
CHR10FS100051523 0.0789348
CHR10FS100051638 -0.0729561
CHR10FS100051733 -0.273693
CHR10FS100051818 0.127127
CHR10FS100051928 0.19026
CHR10FS100052019 -0.151852
CHR10FS100052139 -0.301626
CHR10FS100052219 -0.30447
CHR10FS100052334 0.322834
CHR10FS100052429 0.507846
CHR10FS100052519 0.238975
CHR10FS100052639 0.345043
CHR10FS100052720 0.287304
CHR10FS100052868 -0.00852665
CHR10FS100052968 -0.0389683
CHR10FS100053063 -0.124876

Total number of rows: 714900

Table truncated, full table size 18571 Kbytes.




Supplementary file Size Download File type/resource
GSM2431176_71403405_532.pair.gz 12.0 Mb (ftp)(http) PAIR
GSM2431176_71403405_635.pair.gz 11.9 Mb (ftp)(http) PAIR
Processed data included within Sample table
Processed data are available on Series record

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