|
Status |
Public on Dec 17, 2016 |
Title |
B6-1 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Foxp3-ChIP DNA from mouse Treg
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 cell type: CD4+CD25+ Treg gender: male age: 8 weeks chip antibody: anti-Foxp3 antibody (eBioscience cat# 14-4774-82)
|
Growth protocol |
CD4+CD25+ Treg isolation
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA isolation
|
Label |
Cy5 (635)
|
Label protocol |
Nimblegen manufacturer protocol
|
|
|
Channel 2 |
Source name |
Input DNA from CD4+CD25+ Treg
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 cell type: CD4+CD25+ Treg gender: male age: 8 weeks
|
Growth protocol |
CD4+CD25+ Treg isolation
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA isolation
|
Label |
Cy3 (532)
|
Label protocol |
Nimblegen manufacturer protocol
|
|
|
|
Hybridization protocol |
Nimblegen manufacturer protocol
|
Scan protocol |
chip submitted to Nimblegen for scanning
|
Data processing |
Data were analyzed using Partek GS (Partek Inc., St Louis, MO) and the Broad Institutes Interactive Genome Viewer (Version 2.3.32) (Robinson et al., 2011). Raw data underwent Loess normalization, and Student t-testing with a false discovery rate of 0.1 (p-value cutoff 0.0029).
|
|
|
Submission date |
Dec 16, 2016 |
Last update date |
Dec 17, 2016 |
Contact name |
Wayne W Hancock |
Organization name |
CHOP/UPenn
|
Street address |
916B ARC, 3615 Civic Ctr Blvd
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104-4318 |
Country |
USA |
|
|
Platform ID |
GPL21030 |
Series (1) |
GSE92520 |
Foxp3 DNA binding in HDAC6-KO vs wild type Regulatory T cells |
|