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Status
Public on Jan 22, 2017
Title
Aire KO Etopo 1
Sample type
RNA
Source name
Thymus
Organism
Mus musculus
Characteristics
age: 6 Weeks Old Sex: Female strain: C57Bl/6 genotype: Aire-/- cells: MECs treatment: Etopo amplification technique: Ambion WT Expression Kit separation method: Dissection / Trimming
Extracted molecule
total RNA
Extraction protocol
TriZol
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
Hybridization protocol
Ambion WT Expression Kit
Scan protocol
Affymetrix GeneChip Scanner
Data processing
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 16, 2016
Last update date
Jan 22, 2017
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246
Series (2)
GSE92509
Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654
Aire, guardian of immunological tolerance, binds to and activates super-enhancers
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
33.493
10344622
295.7741
10344624
1406.3322
10344633
1776.1909
10344637
682.889
10344653
60.8651
10344658
418.2366
10344674
31.7281
10344679
215.5608
10344707
891.2571
10344713
367.7569
10344723
97.4666
10344725
205.5845
10344741
1235.4155
10344743
77.6035
10344750
1597.4804
10344772
95.1583
10344789
437.6096
10344797
690.3567
10344799
628.5361
Total number of rows: 24922 Table truncated, full table size 433 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2430944_EA07068_371483_MOGENE-1_0-ST-V1_MECHI.AIRE.ETOPO_1.CEL.gz
4.4 Mb
(ftp) (http)
CEL
Processed data included within Sample table