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Sample GSM2430944 Query DataSets for GSM2430944
Status Public on Jan 22, 2017
Title Aire KO Etopo 1
Sample type RNA
 
Source name Thymus
Organism Mus musculus
Characteristics age: 6 Weeks Old
Sex: Female
strain: C57Bl/6
genotype: Aire-/-
cells: MECs
treatment: Etopo
amplification technique: Ambion WT Expression Kit
separation method: Dissection / Trimming
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Ambion WT Expression Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 16, 2016
Last update date Jan 22, 2017
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (2)
GSE92509 Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654 Aire, guardian of immunological tolerance, binds to and activates super-enhancers

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 33.493
10344622 295.7741
10344624 1406.3322
10344633 1776.1909
10344637 682.889
10344653 60.8651
10344658 418.2366
10344674 31.7281
10344679 215.5608
10344707 891.2571
10344713 367.7569
10344723 97.4666
10344725 205.5845
10344741 1235.4155
10344743 77.6035
10344750 1597.4804
10344772 95.1583
10344789 437.6096
10344797 690.3567
10344799 628.5361

Total number of rows: 24922

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM2430944_EA07068_371483_MOGENE-1_0-ST-V1_MECHI.AIRE.ETOPO_1.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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