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Status
Public on Jan 22, 2017
Title
WT Topo 2
Sample type
RNA
Source name
Thymus
Organism
Mus musculus
Characteristics
age: 6 Weeks Old Sex: Male strain: C57Bl/6 genotype: Aire+/+ cells: MECs treatment: Topo amplification technique: Ambion WT Expression Kit separation method: Dissection / Trimming
Extracted molecule
total RNA
Extraction protocol
TriZol
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
Hybridization protocol
Ambion WT Expression Kit
Scan protocol
Affymetrix GeneChip Scanner
Data processing
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 16, 2016
Last update date
Jan 22, 2017
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246
Series (2)
GSE92509
Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654
Aire, guardian of immunological tolerance, binds to and activates super-enhancers
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
30.8484
10344622
334.8486
10344624
1381.335
10344633
1377.2431
10344637
530.3224
10344653
60.6832
10344658
412.7914
10344674
29.4492
10344679
271.2259
10344707
726.0165
10344713
509.2049
10344723
82.016
10344725
169.7918
10344741
729.7247
10344743
87.4097
10344750
907.6838
10344772
76.4798
10344789
407.8511
10344797
636.6404
10344799
462.0691
Total number of rows: 24922 Table truncated, full table size 433 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2430939_EA07068_371490_MOGENE-1_0-ST-V1_MECHI.WT.TOPO_4.CEL.gz
4.5 Mb
(ftp) (http)
CEL
Processed data included within Sample table