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Sample GSM2430939 Query DataSets for GSM2430939
Status Public on Jan 22, 2017
Title WT Topo 2
Sample type RNA
 
Source name Thymus
Organism Mus musculus
Characteristics age: 6 Weeks Old
Sex: Male
strain: C57Bl/6
genotype: Aire+/+
cells: MECs
treatment: Topo
amplification technique: Ambion WT Expression Kit
separation method: Dissection / Trimming
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Ambion WT Expression Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 16, 2016
Last update date Jan 22, 2017
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (2)
GSE92509 Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654 Aire, guardian of immunological tolerance, binds to and activates super-enhancers

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 30.8484
10344622 334.8486
10344624 1381.335
10344633 1377.2431
10344637 530.3224
10344653 60.6832
10344658 412.7914
10344674 29.4492
10344679 271.2259
10344707 726.0165
10344713 509.2049
10344723 82.016
10344725 169.7918
10344741 729.7247
10344743 87.4097
10344750 907.6838
10344772 76.4798
10344789 407.8511
10344797 636.6404
10344799 462.0691

Total number of rows: 24922

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM2430939_EA07068_371490_MOGENE-1_0-ST-V1_MECHI.WT.TOPO_4.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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